Anti-Nitrotyrosine antibody [EM-30] (ab125106)
Key features and details
- Mouse monoclonal [EM-30] to Nitrotyrosine
- Suitable for: WB, IHC-P, IHC-Fr
- Isotype: IgG1
Overview
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Product name
Anti-Nitrotyrosine antibody [EM-30]
See all Nitrotyrosine primary antibodies -
Description
Mouse monoclonal [EM-30] to Nitrotyrosine -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
Immunogen
Chemical/ Small Molecule corresponding to Nitrotyrosine.
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Positive control
- NO2-BSA, NO2-HSA; Human colon cancer epithelium and prostate hyperplasia tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. Purity is > 95% (by SDS-PAGE). -
Clonality
Monoclonal -
Clone number
EM-30 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use a concentration of 10 µg/ml.
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IHC-Fr | (1) |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 10 µg/ml. |
IHC-Fr
Use a concentration of 10 µg/ml. |
Target
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Relevance
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab125106 has been referenced in 6 publications.
- Iannitelli AF et al. The Neurotoxin DSP-4 Dysregulates the Locus Coeruleus-Norepinephrine System and Recapitulates Molecular and Behavioral Aspects of Prodromal Neurodegenerative Disease. eNeuro 10:N/A (2023). PubMed: 36635251
- Batlahally S et al. Soluble Klotho, a biomarker and therapeutic strategy to reduce bronchopulmonary dysplasia and pulmonary hypertension in preterm infants. Sci Rep 10:12368 (2020). PubMed: 32704023
- El-Din EAA et al. Could curcumin ameliorate titanium dioxide nanoparticles effect on the heart? A histopathological, immunohistochemical, and genotoxic study. Environ Sci Pollut Res Int 26:21556-21564 (2019). PubMed: 31127514
- Kim IY et al. The two isoforms of matrix metalloproteinase- 2 have distinct renal spatial and temporal distributions in murine models of types 1 and 2 diabetes mellitus. BMC Nephrol 19:248 (2018). PubMed: 30253743
- Mohamed EM et al. Effect of human umbilical cord blood mesenchymal stem cells administered by intravenous or intravitreal routes on cryo-induced retinal injury. IUBMB Life 69:188-201 (2017). PubMed: 28164440
- Ogunbileje JO et al. Hypermetabolism and hypercatabolism of skeletal muscle accompany mitochondrial stress following severe burn trauma. Am J Physiol Endocrinol Metab 311:E436-48 (2016). PubMed: 27382037