Anti-Nitrotyrosine antibody [EM-30] (ab125106)

Overview

  • Product nameAnti-Nitrotyrosine antibody [EM-30]
    See all Nitrotyrosine primary antibodies
  • Description
    Mouse monoclonal [EM-30] to Nitrotyrosine
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to Nitrotyrosine.

  • Positive control
    • NO2-BSA, NO2-HSA; Human colon cancer epithelium and prostate hyperplasia tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab125106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.

Target

  • RelevanceThe cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.

Anti-Nitrotyrosine antibody [EM-30] images

  • Immunohistochemical analysis of Human tumoral colon epithelium (frozen sections) labelling Nitrotyrosine with ab125106 at 10 µg/mL. 

References for Anti-Nitrotyrosine antibody [EM-30] (ab125106)

ab125106 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

To confirm, the epitope for this antibody is not species specific. In theory, it should detect Nitrotyrosine in all species. However, I have collected the following information for you:

ab125106 Anti-Nitr...

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