The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 0.5µg for 106 cells.
FuncS (In vitro complement-dependent cytotoxicity assays; In vivo depletion and in vitro activation of NK1.1 cells; In vitro redirected lysis): Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays a stimulatory role on natural killer (NK) cells cytotoxicity. Activation by cross-linking of the receptor induces Ca(2+) mobilization and interferon-gamma production.
Tissue specificityExpressed in natural killer cells.