Anti-NK1.1 antibody [PK136] (Allophycocyanin) (ab25352)

Overview

  • Product nameAnti-NK1.1 antibody [PK136] (Allophycocyanin)
    See all NK1.1 primary antibodies
  • Description
    Mouse monoclonal [PK136] to NK1.1 (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Specificityab25352 recognises mouse NK1.1 alloantigen.
  • Tested applicationsSuitable for: Flow Cyt, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    CD mouse spleen and bone marrow cells enriched for NK-1+ cells

Properties

Applications

Our Abpromise guarantee covers the use of ab25352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IP
Functional Studies
  • Application notesFlow Cyt: Use 0.5µg for 106 cells.
    FuncS (In vitro complement-dependent cytotoxicity assays; In vivo depletion and in vitro activation of NK1.1 cells; In vitro redirected lysis): Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a stimulatory role on natural killer (NK) cells cytotoxicity. Activation by cross-linking of the receptor induces Ca(2+) mobilization and interferon-gamma production.
    • Tissue specificityExpressed in natural killer cells.
    • Sequence similaritiesContains 1 C-type lectin domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD161 antigen like family member C antibody
      • CD161 antigen-like family member C antibody
      • CD161c antibody
      • Killer cell lectin-like receptor subfamily B member 1C antibody
      • Klrb1c antibody
      • Klrb1f antibody
      • KLRBC_MOUSE antibody
      • Ly-55c antibody
      • Ly55c antibody
      • Lymphocyte antigen 55c antibody
      • Lymphocyte antigen 59 antibody
      • Natural killer cell surface protein P1-40 antibody
      • NK1.1 antibody
      • NKR P1.9 antibody
      • NKR P1C antibody
      • NKR-P1 40 antibody
      • NKR-P1.9 antibody
      • NKR-P1C antibody
      • NKRP1 antibody
      see all

    References for Anti-NK1.1 antibody [PK136] (Allophycocyanin) (ab25352)

    ab25352 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Mouse Cell (splenocytes)
    Specification splenocytes
    Preparation Cell harvesting/tissue preparation method: cell suspension
    Sample buffer: PBS
    Permeabilization No
    Gating Strategy Live cells
    Username

    Abcam user community

    Verified customer

    Submitted May 19 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"