Anti-NKCC1 antibody (ab99558)

Overview

  • Product name
  • Description
    Goat polyclonal to NKCC1
  • Tested applications
    Suitable for: IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    C-NALQHSIRLSGVEDH

    , corresponding to internal sequence amino acids 769-783 of Human NKCC1 (NP_001037.1.)

  • Positive control
    • WB: Human, rat and mouse skeletal muscle, heart and brain tissue lysates. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab99558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/64000.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
  • Tissue specificity
    Expressed in many tissues.
  • Sequence similarities
    Belongs to the SLC12A transporter family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basolateral Na-K-Cl symporter antibody
    • BSC antibody
    • BSC2 antibody
    • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 antibody
    • mBSC2 antibody
    • MGC104233 antibody
    • NKCC1 antibody
    • PPP1R141 antibody
    • Protein phosphatase 1, regulatory subunit 141 antibody
    • S12A2_HUMAN antibody
    • Slc12a2 antibody
    • sodium-potassium-chloride cotransporter 1 antibody
    • Solute carrier family 12 (sodium/potassium/chloride transporter), member 2 antibody
    • solute carrier family 12 (sodium/potassium/chloride transporters) member 2 antibody
    • Solute carrier family 12 member 2 antibody
    • sy-ns antibody
    see all

Anti-NKCC1 antibody images

  • ICC/IF image of ab99558 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab99558, 5ug/ml) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab99558 (3.8µg/ml) staining of paraffin embedded Human Colon shows membranous staining. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-NKCC1 antibody (ab99558)

ab99558 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Salivary gland)
Specification
Salivary gland
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 03 2014

Thank you for your call today and for letting us know about the trouble with this antibody. As we discussed, I am sending a free of charge replacement vial on the order ***, which should arrive around January 3. Please keep me updated a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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