Anti-NKCC1 antibody (ab99558)

Overview

  • Product nameAnti-NKCC1 antibody
    See all NKCC1 primary antibodies
  • Description
    Goat polyclonal to NKCC1
  • Tested applicationsSuitable for: IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:

    C-NALQHSIRLSGVEDH

    , corresponding to internal sequence amino acids 769-783 of Human NKCC1 (NP_001037.1.)

  • Positive control
    • WB: Human, rat and mouse skeletal muscle, heart and brain tissue lysates. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab99558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/64000.
ICC/IF Use a concentration of 5 µg/ml.

Target

Anti-NKCC1 antibody images

  • ICC/IF image of ab99558 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab99558, 5ug/ml) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab99558 (3.8µg/ml) staining of paraffin embedded Human Colon shows membranous staining. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-NKCC1 antibody (ab99558)

ab99558 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Mouse Tissue sections (Salivary gland)
Specification Salivary gland
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 03 2014

Thank you for your call today and for letting us know about the trouble with this antibody. As we discussed, I am sending a free of charge replacement vial on the order ***, which should arrive around January 3. Please keep me updated a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"