Overview

  • Product name
  • Description
    Mouse polyclonal to NKD2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    NKD2 full length human protein

  • Positive control
    • NKD2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 50 kDa).

Target

  • Function
    Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (By similarity). Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.
  • Tissue specificity
    Expressed in kidney, lung, pancreas and spleen.
  • Sequence similarities
    Belongs to the NKD family.
    Contains 1 EF-hand domain.
  • Developmental stage
    Expressed in fetal kidney and lung.
  • Domain
    The N-terminal domain comprising the first 217 amino acid residues is mostly unstructured.
  • Post-translational
    modifications
    Ubiquitinated, leading to rapid proteasomal degradation. Interaction with TGFA interferes with RNF25 binding and protects against ubiquitination mediated by RNF25.
  • Cellular localization
    Cell membrane. Cytoplasm. Cytoplasmic vesicle.
  • Information by UniProt
  • Database links
  • Alternative names
    • hNkd2 antibody
    • Naked 2 antibody
    • Naked-2 antibody
    • nkd2 antibody
    • NKD2_HUMAN antibody
    • Protein naked cuticle homolog 2 antibody
    see all

Images

  • All lanes : Anti-NKD2 antibody (ab67911) at 1/500 dilution

    Lane 1 : NKD2 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 50 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References

ab67911 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting Abcam. The antibody ab67911 has not been tested in IHC and so I cannot provide any protocol information for you. As it has not been tested in IHC, I can offer you our testing discount. The details of which are below. If there i...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up