Overview

  • Product nameAnti-NKD2 antibody
    See all NKD2 primary antibodies
  • Description
    Mouse polyclonal to NKD2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    NKD2 full length human protein

  • Positive control
    • NKD2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 50 kDa).

Target

  • FunctionCell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (By similarity). Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.
  • Tissue specificityExpressed in kidney, lung, pancreas and spleen.
  • Sequence similaritiesBelongs to the NKD family.
    Contains 1 EF-hand domain.
  • Developmental stageExpressed in fetal kidney and lung.
  • DomainThe N-terminal domain comprising the first 217 amino acid residues is mostly unstructured.
  • Post-translational
    modifications
    Ubiquitinated, leading to rapid proteasomal degradation. Interaction with TGFA interferes with RNF25 binding and protects against ubiquitination mediated by RNF25.
  • Cellular localizationCell membrane. Cytoplasm. Cytoplasmic vesicle.
  • Information by UniProt
  • Database links
  • Alternative names
    • hNkd2 antibody
    • Naked 2 antibody
    • Naked-2 antibody
    • nkd2 antibody
    • NKD2_HUMAN antibody
    • Protein naked cuticle homolog 2 antibody
    see all

Anti-NKD2 antibody images

  • All lanes : Anti-NKD2 antibody (ab67911) at 1/500 dilution

    Lane 1 : NKD2 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 50 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-NKD2 antibody (ab67911)

ab67911 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam. The antibody ab67911 has not been tested in IHC and so I cannot provide any protocol information for you. As it has not been tested in IHC, I can offer you our testing discount. The details of which are below. If there i...

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