Overview

  • Product name
    Anti-NKD2 antibody - C-terminal
    See all NKD2 primary antibodies
  • Description
    Rabbit polyclonal to NKD2 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NKD2 aa 373-401 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q969F2

  • Positive control
    • Jurkat cell line lysates. Human kidney tissue. Jurkat cells. A375 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 50 kDa.
ICC/IF 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (By similarity). Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.
  • Tissue specificity
    Expressed in kidney, lung, pancreas and spleen.
  • Sequence similarities
    Belongs to the NKD family.
    Contains 1 EF-hand domain.
  • Developmental stage
    Expressed in fetal kidney and lung.
  • Domain
    The N-terminal domain comprising the first 217 amino acid residues is mostly unstructured.
  • Post-translational
    modifications
    Ubiquitinated, leading to rapid proteasomal degradation. Interaction with TGFA interferes with RNF25 binding and protects against ubiquitination mediated by RNF25.
  • Cellular localization
    Cell membrane. Cytoplasm. Cytoplasmic vesicle.
  • Information by UniProt
  • Database links
  • Alternative names
    • hNkd2 antibody
    • Naked 2 antibody
    • Naked-2 antibody
    • nkd2 antibody
    • NKD2_HUMAN antibody
    • Protein naked cuticle homolog 2 antibody
    see all

Images

  • Flow cytometric analysis of Jurkat cells labeling NKD2 using ab170804 at a 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used.

  • Immunocytochemistry/ Immunofluorescence analysis of A435  cells labeling NKD2 with ab170804 at a 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

  • Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded Human kidney tissue labeling NKD2 with ab170804 at a 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-NKD2 antibody - C-terminal (ab170804) at 1/100 dilution + Jurkat cell line lysates at 35 µg

    Predicted band size: 50 kDa

References

This product has been referenced in:
  • Zhao S  et al. NKD2, a negative regulator of Wnt signaling, suppresses tumor growth and metastasis in osteosarcoma. Oncogene 34:5069-79 (2015). Read more (PubMed: 25579177) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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