Anti-NKF3 kinase family member antibody (ab121869)

Overview

  • Product name
    Anti-NKF3 kinase family member antibody
    See all NKF3 kinase family member primary antibodies
  • Description
    Rabbit polyclonal to NKF3 kinase family member
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: EPNYESPSSN NQDKDSSQAS KSSIKVPETH KAVLALRLEE KDGKIAVQTE KEESKASTDV AGQAVTINLV PTEEQAKPYR VVNLEQPLCK, corresponding to internal sequence amino acids 384-473 of Human NKF3 kinase family member.

  • Positive control
    • Human stomach tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.
  • Sequence similarities
    Belongs to the protein kinase superfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Autophosphorylated in vitro.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with F-actin in serum-rich medium. Actin colocalization is reduced during serum starvation.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ21140 antibody
    • FLJ34483 antibody
    • KIAA2002 antibody
    • NKF3 kinase family member antibody
    • PEAK1 antibody
    • PEAK1_HUMAN antibody
    • pseudopodium enriched atypical kinase antibody
    • Pseudopodium-enriched atypical kinase 1 antibody
    • SGK269 antibody
    • Sugen kinase 269 antibody
    • Tyrosine-protein kinase SgK269 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab121869 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121869, at 1/1500 dilution, staining NKF3 kinase family member in glandular cells of paraffin-embedded Human stomach tissue by Immunohistochemistry.

References

ab121869 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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