The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionTyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.
Sequence similaritiesBelongs to the protein kinase superfamily. Contains 1 protein kinase domain.
Post-translational modificationsPhosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Autophosphorylated in vitro.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with F-actin in serum-rich medium. Actin colocalization is reduced during serum starvation.
Immunocytochemistry/ Immunofluorescence - Anti-NKF3 kinase family member antibody (ab121869)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab121869 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKF3 kinase family member antibody (ab121869)
ab121869, at 1/1500 dilution, staining NKF3 kinase family member in glandular cells of paraffin-embedded Human stomach tissue by Immunohistochemistry.
References for Anti-NKF3 kinase family member antibody (ab121869)
has not yet been referenced specifically in any publications.
Publishing research using ab121869? Please let us know so that we can cite the reference in this datasheet.
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