Anti-NKG2A antibody (ab96319)
Key features and details
- Rabbit polyclonal to NKG2A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-NKG2A antibody
See all NKG2A primary antibodies -
Description
Rabbit polyclonal to NKG2A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NKG2A (N terminal). The exact sequence is proprietary. Carrier protein conjugated.
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Positive control
- WB: A431 and HepG2 cell lysate. ICC/IF: HeLa cells. IHC-P: H1299 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
Target
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Function
Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. -
Tissue specificity
Natural killer cells. -
Sequence similarities
Contains 1 C-type lectin domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3821 Human
- Omim: 161555 Human
- SwissProt: P26715 Human
- Unigene: 512576 Human
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Alternative names
- C lectin type II protein antibody
- CD159 antigen like family member A antibody
- CD159 antigen-like family member A antibody
see all
Images
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Paraffin-embedded H1299 xenograft stained for NKG2A using ab96319 at a 1/500 dilution in immunohistochemistry.
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15 mins.
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All lanes : Anti-NKG2A antibody (ab96319) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
12% SDS PAGE -
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeled for NKG2A using ab96319 at a 1/200 dilution in immunocytochemistry.
Bottom panel co-stained with Hoechst 33342.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab96319 has not yet been referenced specifically in any publications.