The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 106 cells. ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
RelevanceNKG2A, NKG2C and NKG2E, are isoforms of the NKG2 lectin-like family. In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells.The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa 1 in the mouse. NKG2 is expressed on NK cells, lymphokine-activated killer (LAK) T cells, and some CD8+ memory T cells.