The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.2µg for 106 cells. ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
RelevanceNKG2A, NKG2C and NKG2E, are isoforms of the NKG2 lectin-like family. In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells.The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa 1 in the mouse. NKG2 is expressed on NK cells, lymphokine-activated killer (LAK) T cells, and some CD8+ memory T cells.
References for Anti-NKG2A + C+ E antibody [20d5] (Phycoerythrin) (ab25576)
This product has been referenced in:
Vance RE et al. Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells. J Exp Med190:1801-12 (1999).
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