Anti-NKG2D antibody [1D11] (ab35033)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-NKG2D antibody [1D11]
See all NKG2D primary antibodies -
DescriptionMouse monoclonal [1D11] to NKG2D
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Host speciesMouse
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Specificityab35033 is specific for CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1).
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Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, Functional Studiesmore details
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Species reactivityReacts with: Human
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Immunogen
NKL cells (Human)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone number1D11
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab35033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| Flow Cyt | 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| Functional Studies | Use at an assay dependent concentration. PubMed: 21339333 |
Target
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FunctionReceptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes.
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Tissue specificityNatural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs).
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Sequence similaritiesContains 1 C-type lectin domain.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 100528032 Human
- Entrez Gene: 22914 Human
- Omim: 611817 Human
- SwissProt: P26718 Human
- Unigene: 387787 Human
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Alternative names
- CD314 antibody
- CD314 antigen antibody
- D12S2489E antibody
see all
Images
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![Flow Cytometry - NKG2D antibody [1D11] (ab35033)](http://a.abcam.com/ps/datasheet/images/35/ab35033/NKG2D-Primary-antibodies-ab35033-1.gif)
Flow Cytometry - NKG2D antibody [1D11] (ab35033)ab35033 staining NKG2D in human peripheral blood lymphocytes by Flow Cytometric analysis.
References
This product has been referenced in:
- Fernández L et al. In vitro Natural Killer Cell Immunotherapy for Medulloblastoma. Front Oncol 3:94 (2013). Flow Cyt ; Human . Read more (PubMed: 23626949) »
- Abeyweera TP et al. Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells. J Cell Biol 192:675-90 (2011). Functional Studies ; Human . Read more (PubMed: 21339333) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"