Customer reviews and FAQs

Overview

  • Product name
    Anti-NKG2D antibody [1D11]
    See all NKG2D primary antibodies
  • Description
    Mouse monoclonal [1D11] to NKG2D
  • Specificity
    ab35033 is specific for CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1).
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    NKL cells (Human)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    1D11
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab35033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. PubMed: 21339333

Target

  • Function
    Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes.
  • Tissue specificity
    Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs).
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • CD314 antibody
      • CD314 antigen antibody
      • D12S2489E antibody
      • Killer cell lectin like receptor subfamily K member 1 antibody
      • Killer cell lectin-like receptor subfamily K member 1 antibody
      • KLR antibody
      • KLRC4 KLRK1 readthrough antibody
      • KLRK1 antibody
      • NK cell receptor D antibody
      • NK lectin-like receptor antibody
      • NKG2 D activating NK receptor antibody
      • NKG2 D type II integral membrane protein antibody
      • NKG2-D antibody
      • NKG2-D type II integral membrane protein antibody
      • NKG2-D-activating NK receptor antibody
      • Nkg2d antibody
      • NKG2D_HUMAN antibody
      • NKLLR antibody
      • NKR P2 antibody
      • Nkrp2 antibody
      see all

    Anti-NKG2D antibody [1D11] images

    • Flow Cytometry - NKG2D antibody [1D11] (ab35033)
      Flow Cytometry - NKG2D antibody [1D11] (ab35033)
      ab35033 staining NKG2D in human peripheral blood lymphocytes by Flow Cytometric analysis.

    References for Anti-NKG2D antibody [1D11] (ab35033)

    This product has been referenced in:
    • Fernández L  et al. In vitro Natural Killer Cell Immunotherapy for Medulloblastoma. Front Oncol 3:94 (2013). Flow Cyt ; Human . Read more (PubMed: 23626949) »
    • Abeyweera TP  et al. Inhibitory signaling blocks activating receptor clustering and induces cytoskeletal retraction in natural killer cells. J Cell Biol 192:675-90 (2011). Functional Studies ; Human . Read more (PubMed: 21339333) »

    See all 3 Publications for this product

    Publishing research using ab35033? Please let us know so that we can cite the reference in this datasheet.

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    1 Abreview or Q&A

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NKG2D antibody [1D11]

     Poor
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lymph node)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Both Tris-EDTA and Citrate-Sodium
    Specification
    Lymph node
    Blocking step
    (agent) for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
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    Verified customer

    Submitted Aug 12 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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