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Rabbit polyclonal to NKRF
Predicted to work with:
Rabbit, Horse, Dog, Pig, Chimpanzee, Ferret, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 50-100 of Human NKRF (NP_001166959.1).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab168829 was affinity purified using an epitope specific to NKRF immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 78 kDa.
Use at 2-10 µg/mg of lysate.
NKRF is a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. NKRF localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. o involved in the regulation of IL-8 transcription.
NF kappa B repressing factor antibody
NFKB repressing factor antibody
Western blot - Anti-NKRF antibody (ab168829)
All lanes : Anti-NKRF antibody (ab168829) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate Lane 2 : 293T whole cell lysate Lane 3 : Jurkat whole cell lysate Lysates/proteins at 50 µg per lane. Developed using the ECL technique. Predicted band size: 78 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-NKRF antibody (ab168829)
Detection of NKRF in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168829 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"