The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Detects a band of approximately >70, 44 kDa (predicted molecular weight: 78 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceNKRF is a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. NKRF localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. o involved in the regulation of IL-8 transcription.