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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 30 kDa).
Function May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
Sequence similarities Belongs to the NK-2 homeobox family. Contains 1 homeobox DNA-binding domain.
Domain The homeodomain is essential for interaction with OLIG2.
Cellular localization Nucleus.
Information by UniProt
Homeobox protein NK 2 homolog B antibody
Homeobox protein NK-2 homolog B antibody
Homeobox protein Nkx 2.2 antibody
Anti-Nkx2.2 antibody images
Western blot - Nkx2.2 antibody (ab79916)
All lanes : Anti-Nkx2.2 antibody (ab79916) at 1 µg/ml Lane 1 : Human placenta tissue lysate - total protein ( ab29745) Lane 2 : U373 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate ( ab14902) Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 30 kDa Observed band size : 38 kDa ( why is the actual band size different from the predicted?) Additional bands at : 27 kDa,70 kDa,78 kDa. We are unsure as to the identity of these extra bands. Exposure time : 3 minutes
References for Anti-Nkx2.2 antibody (ab79916)
This product has been referenced in:
et al. C-Abl inhibitor imatinib enhances insulin production by ß cells: c-Abl negatively regulates insulin production via interfering with the expression of NKx2.2 and GLUT-2. PLoS One 9:e97694 (2014).
Read more (PubMed: 24835010) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"