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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
Function May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
Sequence similarities Belongs to the NK-2 homeobox family. Contains 1 homeobox DNA-binding domain.
Domain The homeodomain is essential for interaction with OLIG2.
Cellular localization Nucleus.
Information by UniProt
Homeobox protein NK 2 homolog B antibody
Homeobox protein NK-2 homolog B antibody
Homeobox protein Nkx 2.2 antibody
Anti-Nkx2.2 antibody images
Western blot - Anti-Nkx2.2 antibody (ab93188)
All lanes : Anti-Nkx2.2 antibody (ab93188) at 1 µg/ml Lane 1 : Spinal Cord (Mouse) Tissue Lysate Lane 2 : Brain (Mouse) Tissue Lysate Lane 3 : Cerebellum Mouse Tissue Lysate Lane 4 : Mouse Hippocampus Tissue Lysate Lane 5 : Mouse Hippocampus Tissue Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 30 kDa Observed band size : 30 kDa Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands. Exposure time : 2 minutes
References for Anti-Nkx2.2 antibody (ab93188)
has not yet been referenced specifically in any publications.
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