Overview

  • Product nameAnti-Nkx2.2 antibody
    See all Nkx2.2 primary antibodies
  • Description
    Rabbit polyclonal to Nkx2.2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Nkx2.2.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Spinal Cord; Mouse Brain; Mouse Cerebellum; Mouse Hippocampus; Mouse Forebrain.

Properties

Applications

Our Abpromise guarantee covers the use of ab93188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

Target

  • FunctionMay be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance.
  • Sequence similaritiesBelongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • DomainThe homeodomain is essential for interaction with OLIG2.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeobox protein NK 2 homolog B antibody
    • Homeobox protein NK-2 homolog B antibody
    • Homeobox protein Nkx 2.2 antibody
    • Homeobox protein Nkx-2.2 antibody
    • NK 2 homolog B antibody
    • NK2 homeobox 2 antibody
    • NK2 transcription factor like protein B antibody
    • NK2 transcription factor related locus 2 antibody
    • NKX2 2 antibody
    • NKX2-2 antibody
    • NKX22 antibody
    • NKX22_HUMAN antibody
    • Nkx2b antibody
    • tinman antibody
    see all

Anti-Nkx2.2 antibody images

  • All lanes : Anti-Nkx2.2 antibody (ab93188) at 1 µg/ml

    Lane 1 : Spinal Cord (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Cerebellum Mouse Tissue Lysate
    Lane 4 : Mouse Hippocampus Tissue Lysate
    Lane 5 : Mouse Hippocampus Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-Nkx2.2 antibody (ab93188)

ab93188 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"