Overview

  • Product nameAnti-NKX2.3 antibody
    See all NKX2.3 primary antibodies
  • Description
    Rabbit polyclonal to NKX2.3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: EHHFHSAPCM LAAAEGTQFS DGGEEDEEDE GEKLSYLNSL AAADGHGDSG, corresponding to N terminal amino acids 37-86 of Human NKX2.3

  • Positive control
    • Fetal spleen lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • RelevanceThis protein belongs to the NKX family of homeodomain containing transcription factors. These are ivolveded in cell type specification and in the maintenance of differentiated tissue functions.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Homeobox protein NK-2 homolog C antibody
    • homeobox protein NKX2-3 homolog antibody
    • MGC127415 antibody
    • NK-2 (Drosophila) homolog C antibody
    • NK2 transcription factor homolog C antibody
    • NK2 transcription factor related, locus 3 antibody
    • NK2.3 antibody
    • NKX2.3 antibody
    • NKX23 antibody
    • NKX2C antibody
    • tinman antibody
    see all

Anti-NKX2.3 antibody images

  • Anti-NKX2.3 antibody (ab66366) at 0.25 µg/ml + Fetal spleen lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.

References for Anti-NKX2.3 antibody (ab66366)

ab66366 has not yet been referenced specifically in any publications.

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