Overview

  • Product name
    Anti-Nkx2.4 antibody
  • Description
    Rabbit polyclonal to Nkx2.4
  • Host species
    Rabbit
  • Specificity
    ab189202 is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of Nkx2.4 are known to exist; this antibody will detect both isoforms.
  • Tested applications
    Suitable for: ICC, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Nkx2.4 (internal sequence). (18 amino acid peptide).
    Database link: Q9H2Z4

  • Positive control
    • Human skin tissue; A20 cells; A20 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab189202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 2.5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 36 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

Images

  • All lanes : Anti-Nkx2.4 antibody (ab189202) at 1 µg/ml

    Lane 1 : A20 cell lysate
    Lane 2 : A20 cell lysate with immunizing peptide

    Predicted band size: 36 kDa

  • Immunocytochemical analysis of A20 cells labeling Nkx2.4 with ab189202 at 2.5 µg/ml.

  • Immunofluorescent analysis of A20 cells labeling Nkx2.4 with ab189202 at 20 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling Nkx2.4 with ab189202 at 5 µg/ml.

References

ab189202 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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