Anti-Nkx2.5 antibody [2E1] (ab91196)

Overview

  • Product nameAnti-Nkx2.5 antibody [2E1]
    See all Nkx2.5 primary antibodies
  • Description
    Mouse monoclonal [2E1] to Nkx2.5
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment of Human Nkx2.5 expressed in E. Coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab91196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 35 kDa.
ELISA 1/10000.
ICC/IF 1/200 - 1/1000.

Target

  • FunctionImplicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
  • Tissue specificityExpressed only in the heart.
  • Involvement in diseaseAtrial septal defect 7, with or without atrioventricular conduction defects
    Tetralogy of Fallot
    Conotruncal heart malformations
    Hypothyroidism, congenital, non-goitrous, 5
    Ventricular septal defect 3
    Hypoplastic left heart syndrome 2
    Asplenia, isolated congenital
  • Sequence similaritiesBelongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NKX2.5, mouse, homolog of antibody
    • Cardiac-specific homeobox 1 antibody
    • Cardiac-specific homeobox antibody
    • CHNG5 antibody
    • CSX antibody
    • CSX1 antibody
    • FLJ52202 antibody
    • FLJ97166 antibody
    • FLJ97195 antibody
    • FLJ97197 antibody
    • FLJ99536 antibody
    • HLHS2 antibody
    • Homeobox protein CSX antibody
    • Homeobox protein NK-2 homolog E antibody
    • Homeobox protein Nkx 2.5 antibody
    • Homeobox protein Nkx-2.5 antibody
    • NK2 homeobox 5 antibody
    • NK2 transcription factor related locus 5 antibody
    • NK2 transcription factor related, locus 5 (Drosophila) antibody
    • NK2, Drosophila, homolog of, E antibody
    • NKX2-5 antibody
    • NKX2.5 antibody
    • NKX25_HUMAN antibody
    • Nkx2E antibody
    • NKX4-1 antibody
    • Tinman paralog antibody
    • VSD3 antibody
    see all

Anti-Nkx2.5 antibody [2E1] images

  • Anti-Nkx2.5 antibody [2E1] (ab91196) at 1/500 dilution + cell lysate from HEK293 cells transfected with full-length Nkx2.5 (aa 1-324) linked to hIgGFc

    Predicted band size : 35 kDa

References for Anti-Nkx2.5 antibody [2E1] (ab91196)

ab91196 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Sample Human Cell (Cardiac progenitors)
Permeabilization Yes - 0.1% triton
Gating Strategy double positive for NKX2-5 and DAPI and single positive for NKX2-5
Specification Cardiac progenitors
Preparation Cell harvesting/tissue preparation method: HBSS and collagenase II
Sample buffer: PBS
Fixation Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 16 2015

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - 0.05% Saponin
Sample Human Cell (Fetal Heart)
Specification Fetal Heart
Gating Strategy FITC positive NKX2.5 was gated against a negative control of mouse IgG1 isotype followed by same FITC secondary
Preparation Cell harvesting/tissue preparation method: dissociated with MACS tissue dissociation machine
Sample buffer: 10% FBS in PBS
Username

Jennifer Dewing

Verified customer

Submitted Mar 24 2015

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 37°C
Sample Dog Tissue sections (Heart)
Specification Heart
Permeabilization Yes - 0.5% Tween80 PBS wash
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 13 2014

Thank you for contacting us.

The concentration of this antibody ab91196 with lot GR66570-1 is 1 mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Read More

Application Western blot
Sample Rabbit Tissue lysate - whole (heart)
Loading amount 20 µg
Specification heart
Gel Running Conditions Non-reduced Denaturing (10)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Xun Ai

Verified customer

Submitted Oct 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"