Overview

  • Product name
    Anti-Nkx2.5 antibody [2E1]
    See all Nkx2.5 primary antibodies
  • Description
    Mouse monoclonal [2E1] to Nkx2.5
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment of Human Nkx2.5 expressed in E. Coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab91196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 35 kDa.
ELISA 1/10000.
ICC/IF 1/200 - 1/1000.

Target

  • Function
    Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
  • Tissue specificity
    Expressed only in the heart.
  • Involvement in disease
    Atrial septal defect 7, with or without atrioventricular conduction defects
    Tetralogy of Fallot
    Conotruncal heart malformations
    Hypothyroidism, congenital, non-goitrous, 5
    Ventricular septal defect 3
    Hypoplastic left heart syndrome 2
    Asplenia, isolated congenital
  • Sequence similarities
    Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NKX2.5, mouse, homolog of antibody
    • Cardiac-specific homeobox 1 antibody
    • Cardiac-specific homeobox antibody
    • CHNG5 antibody
    • CSX antibody
    • CSX1 antibody
    • FLJ52202 antibody
    • FLJ97166 antibody
    • FLJ97195 antibody
    • FLJ97197 antibody
    • FLJ99536 antibody
    • HLHS2 antibody
    • Homeobox protein CSX antibody
    • Homeobox protein NK-2 homolog E antibody
    • Homeobox protein Nkx 2.5 antibody
    • Homeobox protein Nkx-2.5 antibody
    • NK2 homeobox 5 antibody
    • NK2 transcription factor related locus 5 antibody
    • NK2 transcription factor related, locus 5 (Drosophila) antibody
    • NK2, Drosophila, homolog of, E antibody
    • NKX2-5 antibody
    • NKX2.5 antibody
    • NKX25_HUMAN antibody
    • Nkx2E antibody
    • NKX4-1 antibody
    • Tinman paralog antibody
    • VSD3 antibody
    see all

Images

  • Immunocytochemical analysis of human embryonic stem calls, labeling Nkx2.5 with ab91196. DAPI used to stain nuclei. 

  • Anti-Nkx2.5 antibody [2E1] (ab91196) at 1/500 dilution + cell lysate from HEK293 cells transfected with full-length Nkx2.5 (aa 1-324) linked to hIgGFc

    Predicted band size : 35 kDa

References

This product has been referenced in:
  • Shekhar A  et al. Transcription factor ETV1 is essential for rapid conduction in the heart. J Clin Invest 126:4444-4459 (2016). WB, IHC-Fr ; Mouse . Read more (PubMed: 27775552) »
  • Kim YY  et al. Anti-aging effects of vitamin C on human pluripotent stem cell-derived cardiomyocytes. Age (Dordr) 35:1545-57 (2013). IHC-P . Read more (PubMed: 22843416) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic heart)
Permeabilization
Yes - TritonX100 0.02%
Specification
Embryonic heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Mrs. Sara Ranjbarvaziri

Verified customer

Submitted Mar 03 2017

Application
Flow Cytometry
Sample
Human Cell (Cardiac progenitors)
Permeabilization
Yes - 0.1% triton
Gating Strategy
double positive for NKX2-5 and DAPI and single positive for NKX2-5
Specification
Cardiac progenitors
Preparation
Cell harvesting/tissue preparation method: HBSS and collagenase II
Sample buffer: PBS
Fixation
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 16 2015

Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.05% Saponin
Sample
Human Cell (Fetal Heart)
Specification
Fetal Heart
Gating Strategy
FITC positive NKX2.5 was gated against a negative control of mouse IgG1 isotype followed by same FITC secondary
Preparation
Cell harvesting/tissue preparation method: dissociated with MACS tissue dissociation machine
Sample buffer: 10% FBS in PBS
Username

Jennifer Dewing

Verified customer

Submitted Mar 24 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 37°C
Sample
Dog Tissue sections (Heart)
Specification
Heart
Permeabilization
Yes - 0.5% Tween80 PBS wash
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 13 2014

Thank you for contacting us.

The concentration of this antibody ab91196 with lot GR66570-1 is 1 mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Read More

Application
Western blot
Sample
Rabbit Tissue lysate - whole (heart)
Loading amount
20 µg
Specification
heart
Gel Running Conditions
Non-reduced Denaturing (10)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Xun Ai

Verified customer

Submitted Oct 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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