The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa).
Abcam recommends using 3% milk as the blocking agent.
FunctionImplicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
Tissue specificityExpressed only in the heart.
Involvement in diseaseAtrial septal defect 7, with or without atrioventricular conduction defects Tetralogy of Fallot Conotruncal heart malformations Hypothyroidism, congenital, non-goitrous, 5 Ventricular septal defect 3 Hypoplastic left heart syndrome 2 Asplenia, isolated congenital
Sequence similaritiesBelongs to the NK-2 homeobox family. Contains 1 homeobox DNA-binding domain.
NK2 transcription factor related, locus 5 (Drosophila) antibody
NK2, Drosophila, homolog of, E antibody
Tinman paralog antibody
Anti-Nkx2.5 antibody images
Western blot - Anti-Nkx2.5 antibody (ab106923)
All lanes : Anti-Nkx2.5 antibody (ab106923) at 1 µg/ml (Blocked with 3% Milk)
Lane 1 : Human Nkx2.5 recombinant protein (tagged) at 0.1 µg Lane 2 : HEK293 (Human) Whole Cell Lysate (ab52256) at 20 µg Lane 3 : PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate (negative control) at 20 µg Lane 4 : Heart (Mouse) Mouse Tissue Lysate at 20 µg Lane 5 : Heart (Rat) Tissue Lysate at 20 µg Lane 6 : Human Fetal Heart - Total Protein Tissue Lysate at 20 µg
Secondary Rabbit Anti-Goat IgG H&L (HRP) (ab97100) at 1/20000 dilution Developed using the ECL technique
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab106923 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Western blot - Nkx2.5 antibody (ab106923)
Anti-Nkx2.5 antibody (ab106923) at 0.3 µg/ml + Human heart lysate in RIPA buffer at 35 µg Developed using the ECL technique
Predicted band size : 35 kDa Primary incubation was 1 hour.
The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684).