Anti-Nkx2.5 antibody (ab214296)
Key features and details
- Rabbit polyclonal to Nkx2.5
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Nkx2.5 antibody
See all Nkx2.5 primary antibodies -
Description
Rabbit polyclonal to Nkx2.5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Mouse Nkx2.5 aa 50-150 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P42582 -
Positive control
- IHC-P: Rat heart tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development. -
Tissue specificity
Expressed only in the heart. -
Involvement in disease
Atrial septal defect 7, with or without atrioventricular conduction defects
Tetralogy of Fallot
Conotruncal heart malformations
Hypothyroidism, congenital, non-goitrous, 5
Ventricular septal defect 3
Hypoplastic left heart syndrome 2
Asplenia, isolated congenital -
Sequence similarities
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 18091 Mouse
- Entrez Gene: 114109 Rat
- SwissProt: P42582 Mouse
- SwissProt: O35767 Rat
- Unigene: 41974 Mouse
- Unigene: 6179 Rat
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Alternative names
- NKX2.5, mouse, homolog of antibody
- Cardiac-specific homeobox 1 antibody
- Cardiac-specific homeobox antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab214296 has been referenced in 1 publication.
- Dergilev K et al. C-Kit Cardiac Progenitor Cell Based Cell Sheet Improves Vascularization and Attenuates Cardiac Remodeling following Myocardial Infarction in Rats. Biomed Res Int 2018:3536854 (2018). PubMed: 30046593