Overview

  • Product name
  • Description
    Rabbit polyclonal to Nkx2.5
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 188 of Human Nkx2.5 (NP_004378).

  • Positive control
    • WB: 293T, A431, H1299, HeLaS 3, HepG2, MOLT4 and Raji cell lysates ICC/IF: HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab97355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
  • Tissue specificity
    Expressed only in the heart.
  • Involvement in disease
    Atrial septal defect 7, with or without atrioventricular conduction defects
    Tetralogy of Fallot
    Conotruncal heart malformations
    Hypothyroidism, congenital, non-goitrous, 5
    Ventricular septal defect 3
    Hypoplastic left heart syndrome 2
    Asplenia, isolated congenital
  • Sequence similarities
    Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NKX2.5, mouse, homolog of antibody
    • Cardiac-specific homeobox 1 antibody
    • Cardiac-specific homeobox antibody
    • CHNG5 antibody
    • CSX antibody
    • CSX1 antibody
    • FLJ52202 antibody
    • FLJ97166 antibody
    • FLJ97195 antibody
    • FLJ97197 antibody
    • FLJ99536 antibody
    • HLHS2 antibody
    • Homeobox protein CSX antibody
    • Homeobox protein NK-2 homolog E antibody
    • Homeobox protein Nkx 2.5 antibody
    • Homeobox protein Nkx-2.5 antibody
    • NK2 homeobox 5 antibody
    • NK2 transcription factor related locus 5 antibody
    • NK2 transcription factor related, locus 5 (Drosophila) antibody
    • NK2, Drosophila, homolog of, E antibody
    • NKX2-5 antibody
    • NKX2.5 antibody
    • NKX25_HUMAN antibody
    • Nkx2E antibody
    • NKX4-1 antibody
    • Tinman paralog antibody
    • VSD3 antibody
    see all

Images

  • All lanes : Anti-Nkx2.5 antibody (ab97355) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 35 kDa
  • Immunofluorescence analysis of Nkx2.5 in paraformaldehyde fixed HeLa, using ab97355 at a 1/200 dilution.
    Lower image was merged with DNA probe.

References

This product has been referenced in:
  • Feng Y  et al. Shox2 influences mesenchymal stem cell fate in a co-culture model in vitro. Mol Med Rep 14:637-42 (2016). WB . Read more (PubMed: 27222368) »
  • Chen J  et al. Isolation and characterization of porcine amniotic fluid-derived multipotent stem cells. PLoS One 6:e19964 (2011). ICC . Read more (PubMed: 21625520) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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