Recombinant
RabMAb

Anti-Nkx2.5 antibody [EPR20168] (ab205263)

Overview

  • Product name
    Anti-Nkx2.5 antibody [EPR20168]
    See all Nkx2.5 primary antibodies
  • Description
    Rabbit monoclonal [EPR20168] to Nkx2.5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Nkx2.5. The exact sequence is proprietary.
    Database link: P52952

  • Positive control
    • WB: Human fetal heart muscle tissue lysate; HEK-293T and HeLa whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab205263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 30-42 kDa (predicted molecular weight: 35 kDa).

Target

  • Function
    Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
  • Tissue specificity
    Expressed only in the heart.
  • Involvement in disease
    Atrial septal defect 7, with or without atrioventricular conduction defects
    Tetralogy of Fallot
    Conotruncal heart malformations
    Hypothyroidism, congenital, non-goitrous, 5
    Ventricular septal defect 3
    Hypoplastic left heart syndrome 2
    Asplenia, isolated congenital
  • Sequence similarities
    Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NKX2.5, mouse, homolog of antibody
    • Cardiac-specific homeobox 1 antibody
    • Cardiac-specific homeobox antibody
    • CHNG5 antibody
    • CSX antibody
    • CSX1 antibody
    • FLJ52202 antibody
    • FLJ97166 antibody
    • FLJ97195 antibody
    • FLJ97197 antibody
    • FLJ99536 antibody
    • HLHS2 antibody
    • Homeobox protein CSX antibody
    • Homeobox protein NK-2 homolog E antibody
    • Homeobox protein Nkx 2.5 antibody
    • Homeobox protein Nkx-2.5 antibody
    • NK2 homeobox 5 antibody
    • NK2 transcription factor related locus 5 antibody
    • NK2 transcription factor related, locus 5 (Drosophila) antibody
    • NK2, Drosophila, homolog of, E antibody
    • NKX2-5 antibody
    • NKX2.5 antibody
    • NKX25_HUMAN antibody
    • Nkx2E antibody
    • NKX4-1 antibody
    • Tinman paralog antibody
    • VSD3 antibody
    see all

Images

  • All lanes : Anti-Nkx2.5 antibody [EPR20168] (ab205263) at 1/1000 dilution

    Lane 1 : Human fetal heart muscle tissue lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 30-42 kDa (why is the actual band size different from the predicted?)


    Exposure time: 3 minutes


    Blocking/Dilution: 5% NFDM/TBST

    The molecular masses observed are consistent with what has been described in the literature (PMID: 21447557)

    Negative control: MCF7 (PMID: 15340050).

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

References

ab205263 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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