The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionTranscription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
Tissue specificityHighly expressed in the prostate and, at a lower level, in the testis.
Sequence similaritiesBelongs to the NK-3 homeobox family. Contains 1 homeobox DNA-binding domain.
Post-translational modificationsUbiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues.
Predicted band size : 26 kDa Nkx3.1 antibody (ab55781) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
References for Anti-Nkx3.1 antibody (ab55781)
This product has been referenced in:
Daniels G et al. TBLR1 as an androgen receptor (AR) coactivator selectively activates AR target genes to inhibit prostate cancer growth. Endocr Relat Cancer21:127-42 (2014).
Read more (PubMed: 24243687) »
Bohmer M et al. Structural Analysis of microRNA-Target Interaction by Sequential Seed Mutagenesis and Stem-Loop 3' RACE. PLoS One8:e81427 (2013).
Read more (PubMed: 24282594) »