Overview

  • Product name
  • Description
    Rabbit polyclonal to nkx6.3
  • Specificity
    ab69946 detects endogenous levels of total nkx6.3 protein.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human nkx6.3

  • Positive control
    • HUVEC cells and cell extracts from K562 and COS7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab69946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

Anti-nkx6.3 antibody images

  • ab69946 at 1/500 dilution staining nkx6.3 in HUVEC cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-nkx6.3 antibody (ab69946) at 1/500 dilution

    Lane 1 : K562 cell extract
    Lane 2 : COS7 cell extract
    Lane 3 : K562 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

References for Anti-nkx6.3 antibody (ab69946)

ab69946 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
0.3M glycine, donkey serum, 1% BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Sample
Mouse Cell (adult stomach, embryonic brain)
Specification
adult stomach, embryonic brain
Permeabilization
Yes - 0.5% Triton-X 100
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Sep 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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