Overview

  • Product nameAnti-NLE1 antibody
    See all NLE1 primary antibodies
  • Description
    Rabbit polyclonal to NLE1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human NLE1.

  • Positive control
    • Extracts from COS cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 53 kDa).
ELISA 1/40000.

Target

Anti-NLE1 antibody images

  • All lanes : Anti-NLE1 antibody (ab74143) at 1/500 dilution

    Lane 1 : Extracts from COS cells
    Lane 2 : Extracts from COS cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 53 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

References for Anti-NLE1 antibody (ab74143)

ab74143 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab74143.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"