Overview

  • Product name
  • Description
    Rabbit polyclonal to NLK
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C-terminal amino acids 407-436 of Human NLK (NP_032728.3)

  • Positive control
    • MCF7 cell line and Mouse lung tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab116715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 58 kDa.

Target

  • Function
    Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-303 and Tyr-305, which activates the enzyme.
  • Cellular localization
    Nucleus. Cytoplasm. Predominantly nuclear. A smaller fraction is cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp761G1211 antibody
    • FLJ21033 antibody
    • LAK1 antibody
    • Nemo like kinase antibody
    • Nemo-like kinase antibody
    • Nlk antibody
    • NLK_HUMAN antibody
    • Protein LAK1 antibody
    • Serine/threonine protein kinase NLK antibody
    • Serine/threonine-protein kinase NLK antibody
    see all

Images

  • Anti-NLK antibody (ab116715) at 1/100 dilution + MCF7 cell line lysate at 35 µg

    Predicted band size : 58 kDa
  • Anti-NLK antibody (ab116715) at 1/100 dilution + Mouse lung tissue lysate at 35 µg

    Predicted band size : 58 kDa

References

This product has been referenced in:
  • Lv M  et al. Lentivirus-mediated knockdown of NLK inhibits small-cell lung cancer growth and metastasis. Drug Des Devel Ther 10:3737-3746 (2016). WB ; Human . Read more (PubMed: 27895463) »
  • Tai J  et al. Lentivirus-delivered nemo-like kinase small interfering RNA inhibits laryngeal cancer cell proliferation in vitro. Mol Med Rep 12:5619-24 (2015). WB . Read more (PubMed: 26252054) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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