Anti-NLK antibody (ab26050)
Key features and details
- Rabbit polyclonal to NLK
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NLK antibody
See all NLK primary antibodies -
Description
Rabbit polyclonal to NLK -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Dog, Xenopus laevis -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human NLK.
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Positive control
- This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Mouse Lung Normal; Formalin Fixed Paraffin Embedded Mouse Brain Normal. This antibody gave a positive signal in the following cell types: U-87 MG.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 µg/ml.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsDually phosphorylated on Thr-303 and Tyr-305, which activates the enzyme. -
Cellular localization
Nucleus. Cytoplasm. Predominantly nuclear. A smaller fraction is cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 51701 Human
- Entrez Gene: 18099 Mouse
- Entrez Gene: 497961 Rat
- Omim: 609476 Human
- SwissProt: Q9UBE8 Human
- SwissProt: O54949 Mouse
- SwissProt: D3ZSZ3 Rat
- Unigene: 208759 Human
see all -
Alternative names
- DKFZp761G1211 antibody
- FLJ21033 antibody
- LAK1 antibody
see all
Images
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Anti-NLK was used in standard Vector ABC protocol on paraffin embedded mouse lung with the following modifications: HIER with Vector antigen unmasking solution for 25 minutes at 98C. Used a 1:100 dilution in PBS/Triton-X 4C overnight.
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Adult mouse brain fixed in 10% neutral buffered formulin. Antigen retrieval was performed using Tris-EDTA pH 9.0. The tissue was incubated with ab26050 at a concentration of 2.5µg/ml for 16 hours at 4°C. Nuclear staining can be clearly seen.
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ICC/IF image of ab26050 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab26050 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab26050 has been referenced in 8 publications.
- Dong T et al. Microglia regulate brain progranulin levels through the endocytosis/lysosomal pathway. JCI Insight 6:N/A (2021). PubMed: 34618685
- Daams R et al. Deletion of Nemo-like Kinase in T Cells Reduces Single-Positive CD8+ Thymocyte Population. J Immunol 205:1830-1841 (2020). PubMed: 32839237
- Li X et al. TNF-a regulates the osteogenic differentiation of bone morphogenetic factor 9 adenovirus-transduced rat follicle stem cells via Wnt signaling. Mol Med Rep 22:3141-3150 (2020). PubMed: 32945435
- Song L et al. c-Kit modifies the inflammatory status of smooth muscle cells. PeerJ 5:e3418 (2017). WB ; Mouse . PubMed: 28626608
- Rydell-Törmänen K et al. Aberrant nonfibrotic parenchyma in idiopathic pulmonary fibrosis is correlated with decreased ß-catenin inhibition and increased Wnt5a/b interaction. Physiol Rep 4:N/A (2016). IHC-P ; Human . PubMed: 26997628
- Suwei D et al. NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol 8:120 (2015). Human . PubMed: 26503334
- Shaw-Hallgren G et al. Association of nuclear-localized Nemo-like kinase with heat-shock protein 27 inhibits apoptosis in human breast cancer cells. PLoS One 9:e96506 (2014). IHC . PubMed: 24816797
- Ju H et al. Polyglutamine disease toxicity is regulated by Nemo-like kinase in spinocerebellar ataxia type 1. J Neurosci 33:9328-36 (2013). PubMed: 23719801