Overview

  • Product nameAnti-NLK antibody
    See all NLK primary antibodies
  • Description
    Mouse monoclonal to NLK
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein: DEGRLRYHTC MCKCCFSTST GRVYTSDFEP VTNPKFDDTF EKNLSSVRQV KEIIHQFILE QQKGNRVPLC INPQSAAFKS FISSTVAQPS EMPPSPLVWE , corresponding to amino acids 416-515 of Human NLK

Properties

Applications

Our Abpromise guarantee covers the use of ab57208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Target

  • FunctionRole in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-303 and Tyr-305, which activates the enzyme.
  • Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. A smaller fraction is cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp761G1211 antibody
    • FLJ21033 antibody
    • LAK1 antibody
    • Nemo like kinase antibody
    • Nemo-like kinase antibody
    • Nlk antibody
    • NLK_HUMAN antibody
    • Protein LAK1 antibody
    • Serine/threonine protein kinase NLK antibody
    • Serine/threonine-protein kinase NLK antibody
    see all

Anti-NLK antibody images

  • Western blot against tagged recombinant protein immunogen using ab57208 NLK antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

References for Anti-NLK antibody (ab57208)

ab57208 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Specification HEK293
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 40 µg
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Feb 01 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"