Overview

  • Product name
  • Description
    Rabbit polyclonal to NLK
  • Tested applications
    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 284-500 of Human NLK (AAH64663)

  • Positive control
    • HeLa, A431 and H1299 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-303 and Tyr-305, which activates the enzyme.
  • Cellular localization
    Nucleus. Cytoplasm. Predominantly nuclear. A smaller fraction is cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp761G1211 antibody
    • FLJ21033 antibody
    • LAK1 antibody
    • Nemo like kinase antibody
    • Nemo-like kinase antibody
    • Nlk antibody
    • NLK_HUMAN antibody
    • Protein LAK1 antibody
    • Serine/threonine protein kinase NLK antibody
    • Serine/threonine-protein kinase NLK antibody
    see all

Images

  • Anti-NLK antibody (ab97642) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 58 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97642 at 1/100 dilution. Lower image is co-stained with Hoechst 33342.

References

This product has been referenced in:
  • Huang T  et al. A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways. Nat Commun 7:12885 (2016). Read more (PubMed: 27698350) »
  • Shaw-Hallgren G  et al. Association of nuclear-localized Nemo-like kinase with heat-shock protein 27 inhibits apoptosis in human breast cancer cells. PLoS One 9:e96506 (2014). Read more (PubMed: 24816797) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T cells)
Total protein in input
0.4 µg
Specification
293T cells
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Loading amount
0.4 µg
Specification
293T cells
Gel Running Conditions
Non-reduced Denaturing (7%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Mar 18 2013

Application
Western blot
Sample
Mouse Tissue lysate - whole (Bone Marrow)
Loading amount
0.3 µg
Specification
Bone Marrow
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 15 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa Cells)
Loading amount
20 µg
Specification
HeLa Cells
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted May 17 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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