Overview

  • Product name
  • Description
    Rabbit polyclonal to NLRC3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Human NLRC3.

    (Peptide available as ab90252.)

  • Positive control
    • This antibody gave a positive signal in human lymph node tissue lysate and the following whole cell lysates: Raji; Ramos; Jurkat.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa (predicted molecular weight: 114 kDa).

Target

  • Function
    May modulate T-cell activation. Decreases the transcription of genes that are normally up-regulated after T-cell stimulation. Delays degradation of NFKBIA/IKBA.
  • Tissue specificity
    Detected in peripheral blood mononuclear cells.
  • Sequence similarities
    Belongs to the NLRP family.
    Contains 16 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CARD15-like antibody
    • CARD15-like protein antibody
    • Caterpiller 16.2 antibody
    • Caterpiller protein 16.2 antibody
    • CLR16.2 antibody
    • FLJ00348 antibody
    • NLR family CARD domain containing 3 antibody
    • Nlrc3 antibody
    • NLRC3_HUMAN antibody
    • NOD-like receptor C3 antibody
    • NOD3 antibody
    • NOD3 protein antibody
    • Nucleotide-binding oligomerization domain protein 3 antibody
    • Nucleotide-binding oligomerization domain protein 3 caterpiller 16.2 antibody
    • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3 antibody
    • Protein NLRC3 antibody
    see all

Images

  • All lanes : Anti-NLRC3 antibody (ab77817) at 1 µg/ml

    Lane 1 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 114 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

References

ab77817 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab77817.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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