Overview

  • Product nameAnti-NLRP3 antibody [Nalpy3-a]
    See all NLRP3 primary antibodies
  • Description
    Mouse monoclonal [Nalpy3-a] to NLRP3
  • SpecificityRecognises endogenous human NALP3.
  • Tested applicationsSuitable for: Flow Cyt, ICC, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab16097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC 1/100.
WB 1/1000. Predicted molecular weight: 118 kDa.

Target

  • FunctionMay function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
  • Tissue specificityExpressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
  • Involvement in diseaseDefects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
    Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
    Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation.
  • Sequence similaritiesBelongs to the NLRP family.
    Contains 1 DAPIN domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGTAVPRL antibody
    • AII/AVP antibody
    • Angiotensin/vasopressin receptor AII/AVP like antibody
    • Angiotensin/vasopressin receptor AII/AVP-like antibody
    • C1orf7 antibody
    • Caterpiller protein 1.1 antibody
    • CIAS 1 antibody
    • CIAS1 antibody
    • CLR1.1 antibody
    • Cold autoinflammatory syndrome 1 antibody
    • Cold autoinflammatory syndrome 1 protein antibody
    • Cryopyrin antibody
    • Familial cold autoinflammatory syndrome antibody
    • FCAS antibody
    • FCU antibody
    • LRR and PYD domains-containing protein 3 antibody
    • Muckle-Wells syndrome antibody
    • MWS antibody
    • NACHT antibody
    • NACHT LRR and PYD containing protein 3 antibody
    • NALP 3 antibody
    • NALP3 antibody
    • NALP3_HUMAN antibody
    • NLRP3 antibody
    • PYPAF 1 antibody
    • PYPAF1 antibody
    • PYRIN containing APAF1 like protein 1 antibody
    • PYRIN-containing APAF1-like protein 1 antibody
    see all

Anti-NLRP3 antibody [Nalpy3-a] images

  • Overlay histogram showing Saos 2 cells stained with ab16097 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16097, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-NLRP3 antibody [Nalpy3-a] (ab16097)

This product has been referenced in:
  • Atianand MK  et al. Francisella tularensis Reveals a Disparity between Human and Mouse NLRP3 Inflammasome Activation. J Biol Chem 286:39033-42 (2011). WB ; Human . Read more (PubMed: 21930705) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil)
Specification tonsil
Fixative Formaldehyde
Antigen retrieval step Other
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Submitted Jun 01 2006

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