The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 108 kDa).
FunctionParticipates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF-Kappa-B and type 1 interferon signaling pathways, but enhances NF-Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313).
Tissue specificityUbiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HELA, 293T, THP-1, HL60, RAJI, and JURKAT cell lines (at protein level).
Sequence similaritiesBelongs to the NLRP family. Contains 7 LRR (leucine-rich) repeats. Contains 1 NACHT domain.