The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 108 kDa.
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionParticipates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF-Kappa-B and type 1 interferon signaling pathways, but enhances NF-Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313).
Tissue specificityUbiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HELA, 293T, THP-1, HL60, RAJI, and JURKAT cell lines (at protein level).
Sequence similaritiesBelongs to the NLRP family. Contains 7 LRR (leucine-rich) repeats. Contains 1 NACHT domain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human heart tissue labelling NLRX1 with ab107611 at 10µg/ml. Staining was enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Western blot - NLRX1 antibody (ab107611)
All lanes : Anti-NLRX1 antibody (ab107611) at 5 µg/ml
Lane 1 : Mouse heart tissue lysate in absence immunizing peptide Lane 2 : Mouse heart tissue lysate in presence of immunuzing peptide
Lysates/proteins at 40 µg per lane.
Secondary Goat anti-Rabbit Ig HRP Developed using the ECL technique