This antibody gave a positive signal in the following lysates:
MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate; HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate; THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 mg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 108 kDa).
FunctionParticipates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF-Kappa-B and type 1 interferon signaling pathways, but enhances NF-Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313).
Tissue specificityUbiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HELA, 293T, THP-1, HL60, RAJI, and JURKAT cell lines (at protein level).
Sequence similaritiesBelongs to the NLRP family. Contains 7 LRR (leucine-rich) repeats. Contains 1 NACHT domain.