Anti-NM23A antibody [4B2] (ab98963)

Overview

  • Product nameAnti-NM23A antibody [4B2]
    See all NM23A primary antibodies
  • Description
    Mouse monoclonal [4B2] to NM23A
  • Tested applicationsSuitable for: ELISA, Flow Cyt, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of Human NM23A expressed in E. Coli.

  • Positive control
    • HeLa or Jurkat cell lines; NM23A-hIgGFc transfected HEK293 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab98963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
Flow Cyt 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/2000. Predicted molecular weight: 17 kDa.
ICC/IF 1/200 - 1/1000.

Target

  • FunctionMajor role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.
  • Tissue specificityIsoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed.
  • Sequence similaritiesBelongs to the NDK family.
  • Cellular localizationCytoplasm. Nucleus. Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.
  • Information by UniProt
  • Database links
  • Alternative names
    • AWD antibody
    • AWD, drosophila, homolog of antibody
    • GAAD antibody
    • Granzyme A activated DNase antibody
    • Granzyme A-activated DNase antibody
    • GZMA activated DNase antibody
    • Metastasis inhibition factor NM23 antibody
    • NB antibody
    • NBS antibody
    • NDK A antibody
    • NDKA antibody
    • NDKA_HUMAN antibody
    • NDP kinase A antibody
    • NDPK-A antibody
    • NDPKA antibody
    • NM23 antibody
    • NM23 long variant, included antibody
    • nm23-H1 antibody
    • NM23-M1 antibody
    • NM23H1B, included antibody
    • NME/NM23 nucleoside diphosphate kinase 1 antibody
    • Nme1 antibody
    • NME1-NME2 spliced read-through transcript, included antibody
    • Non-metastatic cells 1, protein (NM23A) expressed in antibody
    • Nonmetastatic cells 1, protein expressed in antibody
    • Nonmetastatic protein 23 antibody
    • Nonmetastatic protein 23, homolog 1 antibody
    • Nucleoside diphosphate kinase A antibody
    • Tumor metastatic process-associated protein antibody
    see all

Anti-NM23A antibody [4B2] images

  • Anti-NM23A antibody [4B2] (ab98963) at 1/500 dilution + NM23A-hIgGFc transfected HEK293 cell lysate

    Predicted band size : 17 kDa
  • Immunofluorescence analysis of Hela cells using 1/200 ab98963 (green). Red: actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Flow cytometric analysis of Jurkat cells at 1:200 using ab98963 (green) and negative control (purple).

References for Anti-NM23A antibody [4B2] (ab98963)

ab98963 has not yet been referenced specifically in any publications.

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