Anti-NM23A antibody (ab80004)

Overview

  • Product nameAnti-NM23A antibody
    See all NM23A primary antibodies
  • Description
    Rabbit polyclonal to NM23A
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human NM23A.

    (Peptide available as ab88971.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HeLa.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab80004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionMajor role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.
  • Tissue specificityIsoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed.
  • Sequence similaritiesBelongs to the NDK family.
  • Cellular localizationCytoplasm. Nucleus. Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.
  • Information by UniProt
  • Database links
  • Alternative names
    • AWD antibody
    • AWD, drosophila, homolog of antibody
    • GAAD antibody
    • Granzyme A activated DNase antibody
    • Granzyme A-activated DNase antibody
    • GZMA activated DNase antibody
    • Metastasis inhibition factor NM23 antibody
    • NB antibody
    • NBS antibody
    • NDK A antibody
    • NDKA antibody
    • NDKA_HUMAN antibody
    • NDP kinase A antibody
    • NDPK-A antibody
    • NDPKA antibody
    • NM23 antibody
    • NM23 long variant, included antibody
    • nm23-H1 antibody
    • NM23-M1 antibody
    • NM23H1B, included antibody
    • NME/NM23 nucleoside diphosphate kinase 1 antibody
    • Nme1 antibody
    • NME1-NME2 spliced read-through transcript, included antibody
    • Non-metastatic cells 1, protein (NM23A) expressed in antibody
    • Nonmetastatic cells 1, protein expressed in antibody
    • Nonmetastatic protein 23 antibody
    • Nonmetastatic protein 23, homolog 1 antibody
    • Nucleoside diphosphate kinase A antibody
    • Tumor metastatic process-associated protein antibody
    see all

Anti-NM23A antibody images

  • All lanes : Anti-NM23A antibody (ab80004) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
    Additional bands at : 250 kDa,40 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 9 minutes
  • ICC/IF image of ab80004 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80004, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-NM23A antibody (ab80004)

ab80004 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam. I just wanted to send you information regarding our 100% Abreview discount program. Please let me know if you have any questions. Thank you very much for your interest in ab8004 and ab98963. To our knowledge, these ant...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (SW1353 chondrosarcoma)
Loading amount 15 µg
Specification SW1353 chondrosarcoma
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Ursula Rodgers

Verified customer

Submitted Jun 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"