Recombinant
RabMAb

Anti-NM23A antibody [EPR3036] (ab92327)

Overview

  • Product name
    Anti-NM23A antibody [EPR3036]
    See all NM23A primary antibodies
  • Description
    Rabbit monoclonal [EPR3036] to NM23A
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human NM23A.

  • Positive control
    • WB: MCF7, A375, HeLa and Jurkat cell lysates IHC-P: Human lung adenocarcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Overnight incubation is recommended.
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.
    • Tissue specificity
      Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed.
    • Sequence similarities
      Belongs to the NDK family.
    • Cellular localization
      Cytoplasm. Nucleus. Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.
    • Information by UniProt
    • Database links
    • Alternative names
      • AWD antibody
      • AWD, drosophila, homolog of antibody
      • GAAD antibody
      • Granzyme A activated DNase antibody
      • Granzyme A-activated DNase antibody
      • GZMA activated DNase antibody
      • Metastasis inhibition factor NM23 antibody
      • NB antibody
      • NBS antibody
      • NDK A antibody
      • NDKA antibody
      • NDKA_HUMAN antibody
      • NDP kinase A antibody
      • NDPK-A antibody
      • NDPKA antibody
      • NM23 antibody
      • NM23 long variant, included antibody
      • nm23-H1 antibody
      • NM23-M1 antibody
      • NM23H1B, included antibody
      • NME/NM23 nucleoside diphosphate kinase 1 antibody
      • Nme1 antibody
      • NME1-NME2 spliced read-through transcript, included antibody
      • Non-metastatic cells 1, protein (NM23A) expressed in antibody
      • Nonmetastatic cells 1, protein expressed in antibody
      • Nonmetastatic protein 23 antibody
      • Nonmetastatic protein 23, homolog 1 antibody
      • Nucleoside diphosphate kinase A antibody
      • Tumor metastatic process-associated protein antibody
      see all

    Images

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NM23A with unpurified ab92327 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • All lanes : Anti-NM23A antibody [EPR3036] (ab92327) at 1/5000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 17 kDa
    • ab92327, at a dilution of 1/100, staining NM23A in formalin fixed, paraffin embedded Human lung adenocarcinoma tissue by Immunohistochemistry. Detection: DAB staining

    References

    ab92327 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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