• Product nameAnti-NM23A antibody [EPR3036]
    See all NM23A primary antibodies
  • Description
    Rabbit monoclonal [EPR3036] to NM23A
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human NM23A.

  • Positive control
    • WB: MCF7, A375, HeLa and Jurkat cell lysates IHC-P: Human lung adenocarcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab92327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Overnight incubation is recommended.
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/100.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionMajor role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.
    • Tissue specificityIsoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed.
    • Sequence similaritiesBelongs to the NDK family.
    • Cellular localizationCytoplasm. Nucleus. Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.
    • Information by UniProt
    • Database links
    • Alternative names
      • AWD antibody
      • AWD, drosophila, homolog of antibody
      • GAAD antibody
      • Granzyme A activated DNase antibody
      • Granzyme A-activated DNase antibody
      • GZMA activated DNase antibody
      • Metastasis inhibition factor NM23 antibody
      • NB antibody
      • NBS antibody
      • NDK A antibody
      • NDKA antibody
      • NDKA_HUMAN antibody
      • NDP kinase A antibody
      • NDPK-A antibody
      • NDPKA antibody
      • NM23 antibody
      • NM23 long variant, included antibody
      • nm23-H1 antibody
      • NM23-M1 antibody
      • NM23H1B, included antibody
      • NME/NM23 nucleoside diphosphate kinase 1 antibody
      • Nme1 antibody
      • NME1-NME2 spliced read-through transcript, included antibody
      • Non-metastatic cells 1, protein (NM23A) expressed in antibody
      • Nonmetastatic cells 1, protein expressed in antibody
      • Nonmetastatic protein 23 antibody
      • Nonmetastatic protein 23, homolog 1 antibody
      • Nucleoside diphosphate kinase A antibody
      • Tumor metastatic process-associated protein antibody
      see all

    Anti-NM23A antibody [EPR3036] images

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NM23A with unpurified ab92327 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • All lanes : Anti-NM23A antibody [EPR3036] (ab92327) at 1/5000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 17 kDa
    • ab92327, at a dilution of 1/100, staining NM23A in formalin fixed, paraffin embedded Human lung adenocarcinoma tissue by Immunohistochemistry. Detection: DAB staining

    References for Anti-NM23A antibody [EPR3036] (ab92327)

    ab92327 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab92327.
    Please use the links above to contact us or submit feedback about this product.