Overview

  • Product name
  • Description
    Goat polyclonal to NMDAR1
  • Specificity
    This antibody is expected to recognise all three reported isoforms (NP_000823.4; NP_067544.1; NP_015566.1).
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Chicken, Dog, Human, Xenopus laevis, Duck, Zebrafish
  • Immunogen

    Synthetic peptide:

    TQERVNNSNKKE

    , corresponding to internal sequence amino acids 486-497 from the internal region of Human NMDAR1 according to NP_000823.4; NP_067544.1; NP_015566.1

  • Positive control
    • Rat brain lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:1,000.
    WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 23 and 105 kDa (predicted molecular weight: 105 kDa).

    The 23kDa band was strong in mouse brain lysates and faint in human brain lysates. The band was successfully blocked by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
    • Sequence similarities
      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
    • Post-translational
      modifications
      NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
    • Cellular localization
      Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
    • Information by UniProt
    • Database links
    • Alternative names
      • GluN1 antibody
      • Glutamate [NMDA] receptor subunit zeta-1 antibody
      • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
      • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
      • glutamate receptor ionotropic, NMDA 1 antibody
      • Grin1 antibody
      • MRD8 antibody
      • N methyl D aspartate receptor antibody
      • N methyl D aspartate receptor channel subunit zeta 1 antibody
      • N methyl D aspartate receptor subunit NR1 antibody
      • N-methyl-D-aspartate receptor subunit NR1 antibody
      • NMD-R1 antibody
      • NMDA 1 antibody
      • NMDA R1 antibody
      • NMDA receptor 1 antibody
      • NMDA1 antibody
      • NMDAR antibody
      • NMDZ1_HUMAN antibody
      • NR1 antibody
      see all

    Anti-NMDAR1 antibody images

    • Anti-NMDAR1 antibody (ab77264) at 2 µg/ml + rat brain lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 105 kDa
      Observed band size : 105 kDa
      Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-NMDAR1 antibody (ab77264)

    This product has been referenced in:
    • Cronican AA  et al. Genome-wide alteration of histone H3K9 acetylation pattern in mouse offspring prenatally exposed to arsenic. PLoS One 8:e53478 (2013). WB ; Mouse . Read more (PubMed: 23405071) »
    • Ghafari M  et al. NMDA-complexes linked to spatial memory performance in the Barnes maze in CD1 mice. Behav Brain Res : (2011). WB ; Mouse . Read more (PubMed: 21377497) »

    See all 2 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Chicken Tissue lysate - whole (Brain)
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Loading amount
    40 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 23 2017

    Thank you for your message and for your patience.
    It is difficult to find three secondary antibodies in three colors that woudl be suitable, but I hope the following will help. Please check the excitation and emission wavelenghts and spectra to en...

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    Thank you for contacting us. We do not have anti GluR1 and GLuR2 antibodies that can be used for Zebrafish. We unfortunately do not have information of GluR1 and GLuR2 protein sequence of Zebrafish so I am sorry we can’t check the homology. How...

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