Anti-NMDAR1 antibody - Neuronal Marker (ab17345)

Overview

  • Product name
    Anti-NMDAR1 antibody - Neuronal Marker
    See all NMDAR1 primary antibodies
  • Description
    Rabbit polyclonal to NMDAR1 - Neuronal Marker
  • Tested applications
    Suitable for: IHC (PFA fixed), WB, IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat NMDAR1.

    (Peptide available as ab17568.)

  • Positive control
    • Rat brain and spinal cord

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed) Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).Can be blocked with Rat NMDAR1 peptide (ab17568).
IHC-FoFr Use at an assay dependent concentration. PubMed: 20831598
ICC/IF 1/1000.

Target

  • Function
    NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
  • Post-translational
    modifications
    NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
  • Cellular localization
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt
  • Database links
  • Alternative names
    • GluN1 antibody
    • Glutamate [NMDA] receptor subunit zeta-1 antibody
    • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
    • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
    • glutamate receptor ionotropic, NMDA 1 antibody
    • Grin1 antibody
    • MRD8 antibody
    • N methyl D aspartate receptor antibody
    • N methyl D aspartate receptor channel subunit zeta 1 antibody
    • N methyl D aspartate receptor subunit NR1 antibody
    • N-methyl-D-aspartate receptor subunit NR1 antibody
    • NMD-R1 antibody
    • NMDA 1 antibody
    • NMDA R1 antibody
    • NMDA receptor 1 antibody
    • NMDA1 antibody
    • NMDAR antibody
    • NMDZ1_HUMAN antibody
    • NR1 antibody
    see all

Anti-NMDAR1 antibody - Neuronal Marker images

  • All lanes : Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/ml

    Lane 1 : HEK293 Whole Cell Lysate Transfected with SHAM
    Lane 2 : HEK293 Whole Cell Lysate Transfected with NR1-1a/NR2A

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 105 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 54 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 secondsNMDAR1 has a predicted molecular weight of 105kDa; however it has a number of potential glycosylation and phosphorylation sites which may affect the migration of the protein (SwissProt data). In addition, a band at 37kDa has been observed (data not shown).
  • ab17345 Rabbit polyclonal to NMDAR1 immunofluorescent staining in the rat brain cortex. Primary antibody ab17345 was incubated at room temperature overnight at 0.5µg/ml. Preincubation with the immunizing peptide (ab17568) resulted in complete blocking of the primary antibody staining. Anti rabbit alexa fluor 488 secondary antibody was used at 1/1000 (incubated for 2h at room temperature). Protocol details: rat tissue was intracardially perfusion fixed with 4% PFA followed by an overnight post fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. As expected NMDAR1 staining is observed in the soma, axon and dendrites of neurons.

  • Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/ml + HEK293 cell lysate co-transfected with NR1 and NR2A recombinant protein at 50 µg

    Predicted band size : 105 kDa
    Observed band size : ~110 kDa (why is the actual band size different from the predicted?)

    HEK293 cells were co-transfected with NMDAR1 (expected MW ~ 105kDa) and NMDAR2A (expected MW ~ 180kDa).  ab17345 NMDAR1 antibody specifically recognises NMDAR1 protein and does not recognise NMDAR2A protein. ab17345 was incubated overnight at 4°C at 2µg/ml.

References for Anti-NMDAR1 antibody - Neuronal Marker (ab17345)

This product has been referenced in:
  • Wang Z  et al. Transient receptor potential channel 1/4 reduces subarachnoid hemorrhage-induced early brain injury in rats via calcineurin-mediated NMDAR and NFAT dephosphorylation. Sci Rep 6:33577 (2016). Read more (PubMed: 27641617) »
  • Bach EC  et al. Enhanced NMDA receptor-mediated modulation of excitatory neurotransmission in the dorsal vagal complex of streptozotocin-treated, chronically hyperglycemic mice. PLoS One 10:e0121022 (2015). Read more (PubMed: 25799386) »

See all 8 Publications for this product

Product Wall

Thank you for contacting us.

We have a few rabbit-raised antibodies to NMDAR1 that were tested in mouse as well as WB and ICC.

ab28669 (http://www.abcam.com/NMDAR1-antibody-ab28669.html), but based on the immunogen sequence, it wou...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary Neurons Cortex)
Specification
Primary Neurons Cortex
Fixative
Formaldehyde
Permeabilization
Yes - Methanol
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Apr 11 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 07 2007

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (hippocampus and cortex)
Specification
hippocampus and cortex
Fixative
Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

Submitted Apr 12 2006

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