Overview

  • Product nameAnti-NMDAR1 C1 antibody
    See all NMDAR1 C1 primary antibodies
  • Description
    Rabbit polyclonal to NMDAR1 C1
  • SpecificitySpecific to NMDAR1 C1. The specificity is defined based on ab6484's specific immunolabelling of the 100 kDa band in Western blots of rat hippocampal homogenate at antibody dilution of 1:2000.
  • Tested applicationsSuitable for: IHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Raised against the peptide representing the sequence of the C1 splice variant.

  • Positive control
    • Rat hippocampal homogenate.
  • General notes


    The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesThe ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/2000.

A band is seen at ~100 kDa representing the NMDAR1 C1 splice variant.

IP Use at an assay dependent concentration.

Target

  • FunctionNMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
  • Involvement in diseaseDefects in GRIN1 are the cause of mental retardation autosomal dominant type 8 (MRD8) [MIM:614254]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.
  • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
  • Post-translational
    modifications
    NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
  • Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt
  • Database links
  • Alternative names
    • GluN1 antibody
    • Glutamate [NMDA] receptor subunit zeta-1 antibody
    • glutamate receptor, ionotropic, N-methyl D-aspartate 1 antibody
    • Grin1 antibody
    • MRD8 antibody
    • N-methyl-D-aspartate receptor subunit NR1 antibody
    • NMD R1 antibody
    • NMD-R1 antibody
    • NMDA1 antibody
    • Nmdar1 antibody
    • NMDZ1_HUMAN antibody
    • NR1 antibody
    see all

References for Anti-NMDAR1 C1 antibody (ab6484)

This product has been referenced in:
  • Jeffrey RA  et al. Activity-dependent anchoring of importin alpha at the synapse involves regulated binding to the cytoplasmic tail of the NR1-1a subunit of the NMDA receptor. J Neurosci 29:15613-20 (2009). Read more (PubMed: 20016075) »

See 1 Publication for this product

Product Wall

I have been informed that the immunogen used to generate ab6484 is the entire C1 splice variant. Please let me know if you have further questions,

Thank you for your e-mail. This antibody has not been tested against other species than rat and your results suggest that the homology of the immunogen with the mouse protein is too low for the antibody to detect the mouse form. I am in touch with ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"