Anti-NMDAR2A + 2B (phospho Y1246 + Y1252) antibody (ab62436)

Overview

  • Product name
    Anti-NMDAR2A + 2B (phospho Y1246 + Y1252) antibody
  • Description
    Rabbit polyclonal to NMDAR2A + 2B (phospho Y1246 + Y1252)
  • Specificity
    This antibody detects endogenous levels of NMDA or NR2A/B only when phosphorylated at tyrosine 1246/1252.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human NMDA or NR2A/B around the phosphorylation site of tyrosine 1246/1252 (N-L-YP-D-I).

  • Positive control
    • Humna brain tissue; HuvEc cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.

Target

  • Function
    NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • Involvement in disease
    Defects in GRIN2A are the cause of epilepsy with neurodevelopmental defects (EPND) [MIM:613971]. EPND is a neurodevelopmental defect characterized by mental retardation, with behavioral problems, associated with epilepsy, learning difficuties and variable degree of cognitive impairment. Note=A chromosomal aberration involving GRIN2A has been found in a family with epilepsy and neurodevelopmental defects. Translocation t(16;17)(p13.2;q11.2).
    Note=GRIN2A somatic mutations have been frequently found in cutaneous malignant melanoma, suggesting that the glutamate signaling pathway may play a role in the pathogenesis of melanoma (PubMed:21499247).
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • Cellular localization
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GRIN2B antibody
    • Glutamate [NMDA] receptor subunit epsilon 1 antibody
    • Glutamate [NMDA] receptor subunit epsilon 2 antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate [NMDA] receptor subunit epsilon-2 antibody
    • GRIN2A antibody
    • hNR2A antibody
    • hNR3 antibody
    • N methyl D aspartate receptor subtype 2A antibody
    • N methyl D aspartate receptor subtype 2B antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • N-methyl D-aspartate receptor subtype 2B antibody
    • N-methyl-D-aspartate receptor subunit 3 antibody
    • NMDAR2A antibody
    • NMDAR2B antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • NR2B antibody
    • NR3 antibody
    see all

Anti-NMDAR2A + 2B (phospho Y1246 + Y1252) antibody images

  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab62436 at 1/50 dilution, in the presence (right) or absence (left) of immunising phosphopeptide.
  • Immunofluorescent analysis of HuvEc cells using ab62436 at 1/500 dilution, in the presence (right) or absence (left) of immunising phosphopeptide.

References for Anti-NMDAR2A + 2B (phospho Y1246 + Y1252) antibody (ab62436)

ab62436 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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