Overview

  • Product name
  • Description
    Goat polyclonal to NMDAR2A
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis, Chimpanzee
  • Immunogen

    Synthetic peptide:

    C-HIEEKKKSPDF

    , corresponding to amino acids 875-885 of Human NMDAR2A (NP_000824.1).

  • Positive control
    • Human Brain, Cortex and Human Small Intestine tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab118587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3.75 - 7.5 µg/ml.

Target

  • Function
    NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • Cellular localization
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

Images

  • Immunohistochemistry on Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded using ab118587 at a concentration of 7.5 µg/ml.
  • Immunohistochemistry on Human Small Intestine: Formalin-Fixed, Paraffin-Embedded using ab118587 at a concentration of 7.5 µg/ml.

References

This product has been referenced in:
  • Nakai T  et al. Girdin phosphorylation is crucial for synaptic plasticity and memory: a potential role in the interaction of BDNF/TrkB/Akt signaling with NMDA receptor. J Neurosci 34:14995-5008 (2014). WB ; Mouse . Read more (PubMed: 25378165) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Chicken Tissue lysate - whole (Homogenized celebellum of chicken and mouse)
Gel Running Conditions
Non-reduced Denaturing ((4-20% Mini-PROTEAN­ TGX™ Precast Gels))
Loading amount
25 µg
Specification
Homogenized celebellum of chicken and mouse
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mrs. Marthe Fjelldal

Verified customer

Submitted Feb 15 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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