• Product nameAnti-NMDAR2A antibody
    See all NMDAR2A primary antibodies
  • Description
    Rabbit polyclonal to NMDAR2A
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Monkey
  • Immunogen

    Fusion protein (Mouse) (C terminal last 200 amino acids).


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab14596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 170 kDa.
IP Use at an assay dependent dilution.


  • FunctionNMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

Anti-NMDAR2A antibody images

  • Anti-NMDAR2A antibody (ab14596) at 1/1000 dilution + whole cell lysate of synaptosomes of rat hippocampus at 15 µg

    HRP-conjugated goat anti-rabbit antibody at 1/10000 dilution
    Developed using the ECL technique

    Observed band size : 160 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Sophie Pezet

    See Abreview

References for Anti-NMDAR2A antibody (ab14596)

This product has been referenced in:
  • Pochwat B  et al. Antidepressant-like activity of magnesium in the chronic mild stress model in rats: alterations in the NMDA receptor subunits. Int J Neuropsychopharmacol 17:393-405 (2014). WB ; Rat . Read more (PubMed: 24067405) »
  • Buret L & van den Buuse M Corticosterone treatment during adolescence induces down-regulation of reelin and NMDA receptor subunit GLUN2C expression only in male mice: implications for schizophrenia. Int J Neuropsychopharmacol 17:1221-32 (2014). WB ; Mouse . Read more (PubMed: 24556017) »

See all 12 Publications for this product

Product Wall

Application Western blot
Sample Chicken Cell lysate - whole cell (Homogenized chicken embryo cerebellum)
Gel Running Conditions Non-reduced Denaturing (Non-reduced Denaturing (4-20% Mini-PROTEAN­ TGX™ Precast Gels))
Loading amount 25 µg
Specification Homogenized chicken embryo cerebellum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mrs. Marthe Fjelldal

Verified customer

Submitted Mar 24 2017

Thank you for your message and for your patience.
It is difficult to find three secondary antibodies in three colors that woudl be suitable, but I hope the following will help. Please check the excitation and emission wavelenghts and spectra to en...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - other (synaptosomes of rat hippocampus)
Loading amount 15 µg
Specification synaptosomes of rat hippocampus
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Sophie Pezet

Verified customer

Submitted Apr 08 2008