Overview

  • Product name
  • Description
    Rabbit polyclonal to NMDAR2A
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Monkey
  • Immunogen

    Fusion protein (Mouse) (C terminal last 200 amino acids).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 170 kDa.
IP Use at an assay dependent dilution.

Target

  • Function
    NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • Cellular localization
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

Images

  • Anti-NMDAR2A antibody (ab14596) at 1/1000 dilution + whole cell lysate of synaptosomes of rat hippocampus at 15 µg

    Secondary
    HRP-conjugated goat anti-rabbit antibody at 1/10000 dilution
    Developed using the ECL technique

    Observed band size : 160 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Sophie Pezet

    See Abreview

References

This product has been referenced in:
  • Liu E  et al. GSK-3ß deletion in dentate gyrus excitatory neuron impairs synaptic plasticity and memory. Sci Rep 7:5781 (2017). Read more (PubMed: 28720858) »
  • Gonzalez-Lozano MA  et al. Dynamics of the mouse brain cortical synaptic proteome during postnatal brain development. Sci Rep 6:35456 (2016). WB . Read more (PubMed: 27748445) »

See all 18 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Chicken Cell lysate - whole cell (Homogenized chicken embryo cerebellum)
Gel Running Conditions
Non-reduced Denaturing (Non-reduced Denaturing (4-20% Mini-PROTEAN­ TGX™ Precast Gels))
Loading amount
25 µg
Specification
Homogenized chicken embryo cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mrs. Marthe Fjelldal

Verified customer

Submitted Mar 24 2017

Thank you for your message and for your patience.
It is difficult to find three secondary antibodies in three colors that woudl be suitable, but I hope the following will help. Please check the excitation and emission wavelenghts and spectra to en...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - other (synaptosomes of rat hippocampus)
Loading amount
15 µg
Specification
synaptosomes of rat hippocampus
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Sophie Pezet

Verified customer

Submitted Apr 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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