Recombinant
RabMAb

Anti-NMDAR2A antibody [EPR19694] (ab183939)

Overview

  • Product name
    Anti-NMDAR2A antibody [EPR19694]
    See all NMDAR2A primary antibodies
  • Description
    Rabbit monoclonal [EPR19694] to NMDAR2A
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse NMDAR2A aa 1-350. The exact sequence is proprietary.
    Database link: P35436

  • Positive control
    • WB: Mouse and rat hippocampus and brain lysates; His-tagged mouse NMDAR2A recombinant protein fragment (aa aa22-326).
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR19694
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 165 kDa (predicted molecular weight: 165 kDa).

Target

  • Function
    NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • Cellular localization
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

Images

  • All lanes : Anti-NMDAR2A antibody [EPR19694] (ab183939) at 1/1000 dilution

    Lane 1 : Mouse hippocampus lysate
    Lane 2 : Rat hippocampus lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Mouse heart lysate
    Lane 6 : Rat kidney lysate
    Lane 7 : Mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 165 kDa
    Observed band size : 165 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lane 2: 30 seconds; Lane 3, 4, 5, 6 and 7: 3 minutes.

    NMDAR2A has been previously documented to be specifically expressed in neuron cells. (PMID: 12127670).

  • All lanes : Anti-NMDAR2A antibody [EPR19694] (ab183939) at 1/5000 dilution

    Lane 1 : His-tagged mouse NMDAR2A recombinant protein fragment (aa aa22-326)
    Lane 2 : His-tagged mouse NMDAR2B recombinant protein fragment (aa27-322)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 165 kDa
    Observed band size : 165 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 1 seconds; Lane 2: 3 minutes.

     

References

ab183939 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183939.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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