Overview

  • Product nameAnti-NMDAR2A antibody [EPR2465(2)]
    See all NMDAR2A primary antibodies
  • Description
    Rabbit monoclonal [EPR2465(2)] to NMDAR2A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NMDAR2A.

  • Positive control
    • WB: Mouse, rat and human brain tissue lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD) KD = 7.10 x 10 -11 M Learn more about KD
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberEPR2465(2)
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 165 kDa.

Target

  • FunctionNMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

Anti-NMDAR2A antibody [EPR2465(2)] images

  • All lanes : Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/10000 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 165 kDa
    Observed band size : 165 kDa

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/1000 dilution (purified) + Human cerebellum tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 165 kDa
    Observed band size : 165 kDa

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/20000 dilution (purified) + Human fetal brain tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 165 kDa
    Observed band size : 165 kDa

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/1000 dilution (unpurified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Human brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 165 kDa
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-NMDAR2A antibody [EPR2465(2)] (ab124913)

This product has been referenced in:
  • Zhang X  et al. A lasting effect of postnatal sevoflurane anesthesia on the composition of NMDA receptor subunits in rat prefrontal cortex. Int J Dev Neurosci 54:62-69 (2016). WB ; Rat . Read more (PubMed: 27025552) »
  • Chandler JD  et al. Low-dose oral cadmium increases airway reactivity and lung neuronal gene expression in mice. Physiol Rep 4:N/A (2016). WB ; Mouse . Read more (PubMed: 27401458) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"