Anti-NMDAR2A (phospho Y1325) antibody (ab106590)
Key features and details
- Rabbit polyclonal to NMDAR2A (phospho Y1325)
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
-
Product name
Anti-NMDAR2A (phospho Y1325) antibody
See all NMDAR2A primary antibodies -
Description
Rabbit polyclonal to NMDAR2A (phospho Y1325) -
Host species
Rabbit -
Specificity
Specific for the ~180k NMDAR2A protein phosphorylated at Tyr1325 in Western blots. Immunolabelling is completely blocked by preadsorption with the phosphopeptide but not by the corresponding dephosphopeptide. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Dog -
Immunogen
Synthetic peptide corresponding to NMDAR2A (C terminal).
Database link: Q12879 -
Positive control
- Rat hippocampal lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000. Predicted molecular weight: 165 kDa.
|
Notes |
---|
WB
1/1000. Predicted molecular weight: 165 kDa. |
Target
-
Function
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 524212 Cow
- Entrez Gene: 490012 Dog
- Entrez Gene: 14811 Mouse
- Entrez Gene: 24409 Rat
- SwissProt: P35436 Mouse
- SwissProt: Q00959 Rat
- Unigene: 2953 Mouse
- Unigene: 9710 Rat
-
Alternative names
- EPND antibody
- FESD antibody
- GluN2A antibody
see all
Images
-
All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/1000 dilution
Lane 1 : Rat hippocampal lysate
Lane 2 : Rat hippocampal lysate with preadsorption with the phospho-peptide
Predicted band size: 165 kDa
Observed band size: ~180 kDa why is the actual band size different from the predicted? -
All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/500 dilution
All lanes : Rat brain whole tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 165 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab106590 has been referenced in 3 publications.
- Loera-Valencia R et al. High levels of 27-hydroxycholesterol results in synaptic plasticity alterations in the hippocampus. Sci Rep 11:3736 (2021). PubMed: 33580102
- Go BS et al. Glutamatergic neurotransmission in the prefrontal cortex mediates the suppressive effect of intra-prelimbic cortical infusion of BDNF on cocaine-seeking. Eur Neuropsychopharmacol 26:1989-1999 (2016). PubMed: 27765467
- Taniguchi S et al. Involvement of NMDAR2A tyrosine phosphorylation in depression-related behaviour. EMBO J 28:3717-29 (2009). PubMed: 19834457