• Product nameAnti-NMDAR2A (phospho Y1325) antibody
    See all NMDAR2A primary antibodies
  • Description
    Rabbit polyclonal to NMDAR2A (phospho Y1325)
  • SpecificitySpecific for the ~180k NMDAR2A protein phosphorylated at Tyr1325 in Western blots. Immunolabelling is completely blocked by preadsorption with the phosphopeptide but not by the corresponding dephosphopeptide.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Dog
  • Immunogen

    Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1325 of NMDAR2A.

  • Positive control
    • Rat hippocampal lysate


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPrepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab106590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 165 kDa.


  • FunctionNMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

Anti-NMDAR2A (phospho Y1325) antibody images

  • All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/1000 dilution

    Lane 1 : Rat hippocampal lysate
    Lane 2 : Rat hippocampal lysate with preadsorption with the phospho-peptide

    Predicted band size : 165 kDa
    Observed band size : ~180 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/500 dilution

    Lane 1 : Rat brain whole tissue lysate
    Lane 2 : Rat brain whole tissue lysate
    Lane 3 : Rat brain whole tissue lysate
    Lane 4 : Rat brain whole tissue lysate
    Lane 5 : Rat brain whole tissue lysate
    Lane 6 : Rat brain whole tissue lysate
    Lane 7 : Rat brain whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    HRP Goat anti-rabbit IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 165 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Xiaofan Wang

    See Abreview

References for Anti-NMDAR2A (phospho Y1325) antibody (ab106590)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (brain)
Loading amount 20 µg
Specification brain
Gel Running Conditions Reduced Denaturing (3-8% Acetate)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Xiaofan Wang

Verified customer

Submitted Apr 27 2012