• Product nameAnti-NMDAR2A (phospho Y1325) antibody
    See all NMDAR2A primary antibodies
  • Description
    Rabbit polyclonal to NMDAR2A (phospho Y1325)
  • Tested applicationsSuitable for: IHC-P, IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1300 - 1400 of Mouse NMDAR2A, phosphorylated at Y1325.

    (Peptide available as ab16985.)

  • Positive control
    • Mouse brain


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab16646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-FoFr 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 163 kDa (predicted molecular weight: 163 kDa).


  • FunctionNMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

Anti-NMDAR2A (phospho Y1325) antibody images

  • All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab16646) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate with Mouse NMDAR2A (phospho Y1325) peptide (ab16985) at 1 µg/ml
    Lane 3 : Brain (Mouse) Tissue Lysate with Mouse NMDAR2A peptide (ab16990) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 163 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 260 kDa,400 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • Immunohistochemistical detection of NMDAR2A (phospho Y1325) using antibody ab16646 on paraformaldehyde perfusion fixed rat brain sections. Primary Antibody was used at 1/300,  incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: Goat anti-rabbit Alexa Fluor 488 (1/1000). The antibody produced a diffuse staining in the whole CNS; some cellular processes are stained too.

    See Abreview

References for Anti-NMDAR2A (phospho Y1325) antibody (ab16646)

This product has been referenced in:
  • Wu Q  et al. NR2B subunit of the NMDA glutamate receptor regulates appetite in the parabrachial nucleus. Proc Natl Acad Sci U S A 110:14765-70 (2013). Read more (PubMed: 23964123) »
  • Garzón J  et al. RGSZ2 Binds to the Neural Nitric Oxide Synthase PDZ Domain to Regulate Mu-Opioid Receptor-Mediated Potentiation of the N-Methyl-D-Aspartate Receptor-Calmodulin-Dependent Protein Kinase II Pathway. Antioxid Redox Signal 15:873-87 (2011). WB ; Mouse . Read more (PubMed: 21348811) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No

Dr. Sophie Pezet

Verified customer

Submitted Mar 22 2010