Anti-NMDAR2B antibody (ab65783)
Key features and details
- Rabbit polyclonal to NMDAR2B
- Suitable for: WB, ICC/IF, IP, IHC-FoFr
- Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-NMDAR2B antibody
See all NMDAR2B primary antibodies -
Description
Rabbit polyclonal to NMDAR2B -
Host species
Rabbit -
Specificity
Replenishment batches of our polyclonal antibody, ab65783 are tested in WB. Previous batches were additionally validated in ICC/IF, IHC-FoFr and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab254356.
-
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
Predicted to work with: Dog -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1450 to the C-terminus of Rat NMDAR2B.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).
|
ICC/IF |
Use a concentration of 5 µg/ml.
|
|
IP |
Use a concentration of 5 µg/ml.
|
|
IHC-FoFr | (1) |
Use at an assay dependent concentration.
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
IP
Use a concentration of 5 µg/ml. |
IHC-FoFr
Use at an assay dependent concentration. |
Target
-
Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
Tissue specificity
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 417965 Chicken
- Entrez Gene: 494009 Dog
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q5R1P3 Dog
- SwissProt: Q13224 Human
see all -
Alternative names
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
Images
-
ab65783 staining NMDAR2B in Rat Primary Neurons DIV14 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab65783 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown..
-
All lanes : Anti-NMDAR2B antibody (ab65783) at 1 µg/ml
Lane 1 : Rat Hippocampus Tissue Lysate at 10 µg
Lane 2 : Mouse Hippocampus Tissue Lysate at 10 µg
Lane 3 : Human brain tissue lysate - total protein (ab29466) at 20 µg
Lane 4 : Human brain hippocampus tissue lysate - total protein (ab30180) at 20 µg
Lane 5 : Human brain amygdala tissue lysate - total protein at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 200 kDa, 35 kDa, 45 kDa, 56 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab65783 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
-
All lanes : Anti-NMDAR2B antibody (ab65783) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Hippocampus (Mouse) Tissue Lysate
Lane 5 : Rat Hippocampus Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
NMDAR2B contains a number of potential phosphorylation and glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
NMDAR2B was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to NMDAR2B and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65783.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 180kDa; NMDAR2B
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (109)
ab65783 has been referenced in 109 publications.
- Smaga I et al. Intravenous administration of Tat-NR2B9c peptide, a PSD95 inhibitor, attenuates reinstatement of cocaine-seeking behavior in rats. Behav Brain Res 416:113537 (2022). PubMed: 34416299
- Ren D et al. Anterior Cingulate Cortex Mediates Hyperalgesia and Anxiety Induced by Chronic Pancreatitis in Rats. Neurosci Bull 38:342-358 (2022). PubMed: 34907496
- Dyomina AV et al. MTEP, a Selective mGluR5 Antagonist, Had a Neuroprotective Effect but Did Not Prevent the Development of Spontaneous Recurrent Seizures and Behavioral Comorbidities in the Rat Lithium-Pilocarpine Model of Epilepsy. Int J Mol Sci 23:N/A (2022). PubMed: 35008924
- Cheng YC et al. Downregulated Calcium-Binding Protein S100A16 and HSP27 in Placenta-Derived Multipotent Cells Induce Functional Astrocyte Differentiation. Stem Cell Rev Rep 18:839-852 (2022). PubMed: 35061207
- Grochecki P et al. Alteration of Ethanol Reward by Prior Mephedrone Exposure: The Role of Age and Matrix Metalloproteinase-9 (MMP-9). Int J Mol Sci 23:N/A (2022). PubMed: 35216236