For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to NMDAR2B
Predicted to work with:
Mouse, Chicken, Cow, Dog, Human, Non human primates
Synthetic peptide from the N-terminus of rat NMDAR2B.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
Information by UniProt
Glutamate [NMDA] receptor subunit epsilon 2 antibody
Western blot - NMDAR2B antibody (ab73001)
Anti-NMDAR2B antibody (ab73001) at 1/1000 dilution + rat hippocampal lysate at 10 µg
Predicted band size :
Observed band size :
180 kDa (
why is the actual band size different from the predicted?
Additional bands at :
120 kDa,<68 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2B antibody (ab73001)
ab73001 diluted 1/500 staining NMDAR2B on 14DIV cultured rat cortical neurons fixed in methanol at - 20
oC for 10 minutes. Sections were also labelled with an anti-PSD95 antibody in red.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"