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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
Concentration information loading...
Purity Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).
Function NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Tissue specificity Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
Sequence similarities Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
Cellular localization Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
Information by UniProt
Glutamate [NMDA] receptor subunit epsilon 2 antibody
Anti-NMDAR2B antibody images
Western blot - NMDAR2B antibody (ab73001)
Anti-NMDAR2B antibody (ab73001) at 1/1000 dilution + rat hippocampal lysate at 10 µg Predicted band size : 166 kDa Observed band size : 180 kDa ( why is the actual band size different from the predicted?) Additional bands at : 120 kDa,<68 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2B antibody (ab73001)
ab73001 diluted 1/500 staining NMDAR2B on 14DIV cultured rat cortical neurons fixed in methanol at - 20
oC for 10 minutes. Sections were also labelled with an anti-PSD95 antibody in red.
References for Anti-NMDAR2B antibody (ab73001)
This product has been referenced in:
et al. Synaptic and cognitive improvements by inhibition of 2-AG metabolism are through upregulation of microRNA-188-3p in a mouse model of Alzheimer's disease. J Neurosci 34:14919-33 (2014).
Read more (PubMed: 25378159) »
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