- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-NMDAR2B antibody [NR2B]
See all NMDAR2B primary antibodies -
DescriptionMouse monoclonal [NR2B] to NMDAR2B
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SpecificityDetects the N-methyl-D-aspartate (NMDA) receptor type 2B. This antibody has been tested in mouse and rat brain in western blotting. It has not been tested in human for Western blotting - only for ICC on transfected HEK cells.
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Tested applicationsSuitable for: ICC, WB, IP, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment corresponding to Rat NMDAR2B aa 934-1457.
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Positive control
- WB: Mouse brain tissue ICC: transfected HEK cells
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA -
Concentration information loading... -
PurityProtein A purified
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Purification notesProtein A Chromatography
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ClonalityMonoclonal
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Clone numberNR2B
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab28373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 2 µg/ml. Predicted molecular weight: 166 kDa. | |
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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FunctionNMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
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Tissue specificityPrimarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
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Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
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Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
Alternative names
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
Anti-NMDAR2B antibody [NR2B] images
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![Western blot - NMDAR2B antibody [NR2B] (ab28373)](http://a.abcam.com/ps/datasheet/Images/28/ab28373/ab28372-2.png)
Western blot - NMDAR2B antibody [NR2B] (ab28373)Anti-NMDAR2B antibody [NR2B] (ab28373) at 2 µg/ml + mouse brain tissue
Predicted band size : 166 kDa -
![Western blot - NMDAR2B antibody [NR2B] (ab28373)](http://a.abcam.com/ps/datasheet/Images/28/ab28373/ab28373_1.jpg)
Western blot - NMDAR2B antibody [NR2B] (ab28373)Lanes 2 - 4 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution
Lanes 5 - 7 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/1000 dilution
Lane 2 : As above
Lane 3 : Mouse brain (50ug)
Lane 4 : Rat brain (50ug)
Lane 5 : As above
Lane 6 : Mouse brain (50ug)
Lane 7 : Rat brain (50ug)
Predicted band size : 166 kDa -
](http://a.abcam.com/ps/datasheet/images/28/ab28373/NMDAR2B-Primary-antibodies-ab28373-12.jpg)
Flow Cytometry-Anti-NMDAR2B antibody [NR2B](ab28373)Overlay histogram showing SH-SY5Y cells stained with ab28373 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28373, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
References for Anti-NMDAR2B antibody [NR2B] (ab28373)
This product has been referenced in:
- Posa L et al. Methadone Reverses Analgesic Tolerance Induced by Morphine Pretreatment. Int J Neuropsychopharmacol 19:N/A (2016). WB ; Mouse . Read more (PubMed: 26390873) »
- Gómez-Galán M et al. Running Opposes the Effects of Social Isolation on Synaptic Plasticity and Transmission in a Rat Model of Depression. PLoS One 11:e0165071 (2016). Read more (PubMed: 27764188) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"