Overview

  • Product name
    Anti-NMDAR2B antibody [NR2B]
    See all NMDAR2B primary antibodies
  • Description
    Mouse monoclonal [NR2B] to NMDAR2B
  • Host species
    Mouse
  • Specificity
    Detects the N-methyl-D-aspartate (NMDA) receptor type 2B. This antibody has been tested in mouse and rat brain in western blotting. It has not been tested in human for Western blotting - only for ICC on transfected HEK cells.
  • Tested applications
    Suitable for: ICC, WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Rat NMDAR2B aa 934-1457.

  • Positive control
    • WB: Mouse brain tissue ICC: transfected HEK cells

Properties

Applications

Our Abpromise guarantee covers the use of ab28373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 1 µg/ml.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 166 kDa.
IHC-P 1/10 - 1/100.
Flow Cyt Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
  • Tissue specificity
    Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
  • Cellular localization
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • AW490526 antibody
    • EIEE27 antibody
    • Glutamate [NMDA] receptor subunit epsilon 2 antibody
    • Glutamate [NMDA] receptor subunit epsilon-2 antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
    • Glutamate receptor ionotropic NMDA2B antibody
    • Glutamate receptor subunit epsilon 2 antibody
    • Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
    • GRIN 2B antibody
    • GRIN2B antibody
    • hNR 3 antibody
    • hNR3 antibody
    • MGC142178 antibody
    • MGC142180 antibody
    • MRD6 antibody
    • N methyl D asparate receptor channel subunit epsilon 2 antibody
    • N methyl D aspartate receptor subtype 2B antibody
    • N methyl D aspartate receptor subunit 2B antibody
    • N methyl D aspartate receptor subunit 3 antibody
    • N-methyl D-aspartate receptor subtype 2B antibody
    • N-methyl-D-aspartate receptor subunit 3 antibody
    • NMDA NR2B antibody
    • NMDA R2B antibody
    • NMDAR2B antibody
    • NMDE2 antibody
    • NMDE2_HUMAN antibody
    • NME2 antibody
    • NR2B antibody
    • NR3 antibody
    see all

Images

  • Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Anti-NMDAR2B antibody [NR2B] (ab28373) at 2 µg/ml + mouse brain tissue

    Predicted band size: 166 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [NR2B] (ab28373)

    ab28373 staining NMDAR2B in Human breast tissue sections (right) compared with negative control (left) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% H2O2-methanol for 15 minutes at room temperature; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/20) overnight at 4°C. A HRP-conjugated secondary antibody was used for detection.

  • Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Lanes 2-4 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution
    Lanes 5-7 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/1000 dilution

    Lanes 3 & 6 : Mouse brain (50ug)
    Lanes 4 & 7 : Rat brain (50ug)

    Predicted band size: 166 kDa

  • Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373)This image is courtesy of an anonymous Abreview
    Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution + Rat P14 Cortex Lysate, vesicular fraction at 5 µg

    Secondary
    HRP conjugated Donkey anti-mouse IgG at 1/15000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 166 kDa



    Samples were incubated with primary antibody for 12 hours at 4ºC.

    Blocked with 5% milk for 1 hour at 22ºC. 

    See Abreview

  • Flow Cytometry - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Flow Cytometry - Anti-NMDAR2B antibody [NR2B] (ab28373)
    Overlay histogram showing SH-SY5Y cells stained with ab28373 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28373, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Posa L  et al. Methadone Reverses Analgesic Tolerance Induced by Morphine Pretreatment. Int J Neuropsychopharmacol 19:N/A (2016). WB ; Mouse . Read more (PubMed: 26390873) »
  • Gómez-Galán M  et al. Running Opposes the Effects of Social Isolation on Synaptic Plasticity and Transmission in a Rat Model of Depression. PLoS One 11:e0165071 (2016). Read more (PubMed: 27764188) »

See all 5 Publications for this product

Publishing research using ab28373? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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6 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-NMDAR2B antibody [NR2B]

 Poor
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
Sample
Rat Tissue lysate - other (hippocampi total membrane fraction)
Specification
hippocampi total membrane fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
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Abcam user community

Verified customer

Submitted Feb 19 2014

Dear Abcam manager


I just send you the mail again to ask about the 2nd antibody for fluoroscence WB detection.

I sent you the specification information on chemidoc system that we want to use last week but didn't get reply ye...

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Thank you for your message and for your patience.
It is difficult to find three secondary antibodies in three colors that woudl be suitable, but I hope the following will help. Please check the excitation and emission wavelenghts and spectra to en...

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Currently using ab28373 in hamster tissue (IHC-Fr Fl), but not getting any signal.
Is there any NR2B antibodies that would work with hamster tissue.

Thank you for calling Abcam earlier today.

I have continued to try and find the hamster sequence for NR2B to see if there is any antibodies in our catalogue that may work for hamster, but I have not been able to find the hamster sequence.
...

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Would like to try this antibody with hamster samples.

Thank you for your call today. I've issued the discount code below for testing ab28373in hamster samples.

DISCOUNT CODE: ***
Expiration date: July 30, 2012

This code will give you 1 free primary antibody before the expiration d...

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Immunocytochemistry abreview for Anti-NMDAR2B antibody [NR2B]

 Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Hippocampal neurons)
Specification
Hippocampal neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
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Abcam user community

Verified customer

Submitted Dec 23 2011

Western blot abreview for Anti-NMDAR2B antibody [NR2B]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat P14 Cortex Lysate, vesicular fraction)
Loading amount
5 µg
Specification
Rat P14 Cortex Lysate, vesicular fraction
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Dec 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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