Recombinant fragment corresponding to Rat NMDAR2B aa 934-1457.
Our Abpromise guarantee covers the use of ab28373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC | Use a concentration of 1 µg/ml. | |
WB | Use a concentration of 2 µg/ml. Predicted molecular weight: 166 kDa. | |
IHC-P | 1/10 - 1/100. | |
Flow Cyt | Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
ab28373 staining NMDAR2B in Human breast tissue sections (right) compared with negative control (left) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% H2O2-methanol for 15 minutes at room temperature; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/20) overnight at 4°C. A HRP-conjugated secondary antibody was used for detection.
Samples were incubated with primary antibody for 12 hours at 4ºC.
Blocked with 5% milk for 1 hour at 22ºC.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"