Overview

  • Product nameAnti-NMDAR2B antibody [NR2B]
    See all NMDAR2B primary antibodies
  • Description
    Mouse monoclonal [NR2B] to NMDAR2B
  • SpecificityDetects the N-methyl-D-aspartate (NMDA) receptor type 2B. This antibody has been tested in mouse and rat brain in western blotting. It has not been tested in human for Western blotting - only for ICC on transfected HEK cells.
  • Tested applicationsSuitable for: ICC, WB, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Rat NMDAR2B aa 934-1457.

  • Positive control
    • WB: Mouse brain tissue ICC: transfected HEK cells

Properties

Applications

Our Abpromise guarantee covers the use of ab28373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 1 µg/ml.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 166 kDa.
IP Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionNMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
  • Tissue specificityPrimarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
  • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
  • Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW490526 antibody
    • EIEE27 antibody
    • Glutamate [NMDA] receptor subunit epsilon 2 antibody
    • Glutamate [NMDA] receptor subunit epsilon-2 antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
    • Glutamate receptor ionotropic NMDA2B antibody
    • Glutamate receptor subunit epsilon 2 antibody
    • Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
    • GRIN 2B antibody
    • GRIN2B antibody
    • hNR 3 antibody
    • hNR3 antibody
    • MGC142178 antibody
    • MGC142180 antibody
    • MRD6 antibody
    • N methyl D asparate receptor channel subunit epsilon 2 antibody
    • N methyl D aspartate receptor subtype 2B antibody
    • N methyl D aspartate receptor subunit 2B antibody
    • N methyl D aspartate receptor subunit 3 antibody
    • N-methyl D-aspartate receptor subtype 2B antibody
    • N-methyl-D-aspartate receptor subunit 3 antibody
    • NMDA NR2B antibody
    • NMDA R2B antibody
    • NMDAR2B antibody
    • NMDE2 antibody
    • NMDE2_HUMAN antibody
    • NME2 antibody
    • NR2B antibody
    • NR3 antibody
    see all

Anti-NMDAR2B antibody [NR2B] images

  • Anti-NMDAR2B antibody [NR2B] (ab28373) at 2 µg/ml + mouse brain tissue

    Predicted band size : 166 kDa
  • Lanes 2 - 4 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution
    Lanes 5 - 7 : Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/1000 dilution

    Lane 2 : As above
    Lane 3 : Mouse brain (50ug)
    Lane 4 : Rat brain (50ug)
    Lane 5 : As above
    Lane 6 : Mouse brain (50ug)
    Lane 7 : Rat brain (50ug)


    Predicted band size : 166 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab28373 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28373, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-NMDAR2B antibody [NR2B] (ab28373)

This product has been referenced in:
  • Posa L  et al. Methadone Reverses Analgesic Tolerance Induced by Morphine Pretreatment. Int J Neuropsychopharmacol 19:N/A (2016). WB ; Mouse . Read more (PubMed: 26390873) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
Sample Rat Tissue lysate - other (hippocampi total membrane fraction)
Specification hippocampi total membrane fraction
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 19 2014

Thank you for your message and for your patience.
It is difficult to find three secondary antibodies in three colors that woudl be suitable, but I hope the following will help. Please check the excitation and emission wavelenghts and spectra to en...

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Thank you for calling Abcam earlier today.

I have continued to try and find the hamster sequence for NR2B to see if there is any antibodies in our catalogue that may work for hamster, but I have not been able to find the hamster sequence.
...

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Thank you for your call today. I've issued the discount code below for testing ab28373in hamster samples.

DISCOUNT CODE: ***
Expiration date: July 30, 2012

This code will give you 1 free primary antibody before the expiration d...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (Hippocampal neurons)
Specification Hippocampal neurons
Fixative Paraformaldehyde
Permeabilization Yes - 0.25% Triton in PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
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Verified customer

Submitted Dec 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Rat P14 Cortex Lysate, vesicular fraction)
Loading amount 5 µg
Specification Rat P14 Cortex Lysate, vesicular fraction
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"